高效液相色谱法同时测定红药胶囊中6种有效成分含量

目的:建立高效液相色谱测定红药胶囊中6种成分三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1、欧前胡素、异欧前胡素和羟基红花黄色素A的含量。方法:采用Waster XTerra C18色谱柱(250×4.6 mm,5μm),流动相为乙腈-0.1%磷酸水溶液,流速1.0 m L/min,检测波长为203 nm、300 nm和403 nm,柱温30℃。结果:三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1、欧前胡素、异欧前胡素和羟基红花黄色素A的进样量分别在0.310~5.425μg(0.9997),0.404~7.070μg(0.9998),0.420~7.350μg(0.9997),0.008~0.140μg(0.9996),0.002~0.034μg(0.9996),0.012~0.217μg(0.9997)范围内与峰面积线性关系良好,平均加样回收率(n=6)分别为98.23%、99.15%、100.32%、99.91%,98.67%,99.27%。结论:含量测定方法可作为红药胶囊的质量控制的有效方法。

High Performance Liquid Chromatography Method for Simultaneous Determination of Six Kinds of Active Ingredients Contents in Hongyao Capsules

Objective:To establish an High Performance Liquid Chromatography (HPLD) method for simultaneous determination of notoginsenoside R1,ginsenoside Rg1,ginsenoside Rb1,imperatorin,isoimperatorin,Hydroxy safflower yellow A (HSYA) contents in Hongyao capsules.Methods:Use the Waster XTerraC18 chromatographic column (250 × 4.6 rim),with acctonitrile-0.1％ phosphoric acid gradient elution as mobile phrase,at a flow rate of 1.0 mL/min,detection wavelength of 203 nm,300 nm and 403 nm and column temperature of 30℃.Results:Good linear relationship were observed between sample sizes and the peak areas of notoginsenoside R1,ginsenoside Rg1,ginsenoside Rb1,imperatorin,isoimperatorin,Hydroxy safflower yellow A (HSYA) in the ranges of 0.310 ～ 5.425 μg (0.9997),0.404 ～ 7.070 μg (0.9998),0.420 ～ 7.350 μg (0.9997),0.008 ～ 0.140 μg (0.9996),0.002 ～0.034 μg (0.9996),0.012 ～ 0.217 μg (0.9997) μg respectively,the average recovery rates (n =6) were 8.23％,99.15％,100.32％,99.91％,98.67％,99.27％ respectively.Conclusion:The established quantification method is applicable for the quality control of Hongyao capsules.