Box-Behnken响应面法优选大孔树脂分离纯化三七总皂苷的工艺研究

目的:优选大孔树脂分离纯化三七总皂苷的工艺条件及参数。方法:通过静态吸附法优选大孔树脂型号,以上样生药质量浓度、乙醇洗脱浓度、乙醇洗脱用量为自变量,总皂苷吸附-解析率为因变量,采用单因素试验和Box-Behnken响应面法优选大孔树脂分离纯化三七总皂苷的工艺条件。结果:最佳纯化工艺参数为上样生药质量浓度0.4 g/m L,乙醇洗脱浓度65%,乙醇洗脱用量5.5 BV,此条件下总皂苷的吸附-解析率为94.66%,与理论预测值基本吻合。结论:优化工艺稳定、可行,具有一定的实用价值。

Optimizing Macroporous Resin Purification Technology of Total Saponins from Panax Notoginsen by Box-Behnken Response Surface

Objective:to optimize purification technology of total saponins from panax notoginsen by macroporous resin.Methods:The type of macroporous resins was optimized by static adsorption method,the concentration of sample solution,ethanol concentration and volume as independent variables,to optimize macroporous resin purification technology of total saponins from panax notoginsen by single factor experiment and the Box-Behnken response surface.Results:the best purification technology was as following the concentration of sample solution 0.42g/mL,ethanol concentration 64.74％ and volume 5BV,with the condition that total saponins adsorption-resolution 96.％,the experimental values agreed with those predicted.Conclusion:It was stable and feasible for this purification technology,and it has oractical value.