Suppression of PRDX4 inhibits cell proliferation and invasion of ectopic endometrial stromal cells in endometriosis |
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| Authors: | Yong Luo Yang Zou Li-ping Li Zhao-zhen Wang Li-qun Wang Zi-yu Zhang |
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| Affiliation: | 1. Key Laboratory of Women’s Reproductive Health of Jiangxi Province, Jiangxi Provincial Maternal and Child Health Hospital, Nanchang, Jiangxi, China;2. Central Laboratory, Jiangxi Provincial Maternal and Child Health Hospital, Nanchang, Jiangxi, China;3. Prenatal Diagnosis Center, Jiangxi Provincial Maternal and Child Health Hospital, Nanchang, Jiangxi, China;4. Department of Clinical Medicine, Jiangxi Health Vocational College, Nanchang, Jiangxi, China;5. Department of Reproductive Health, Jiangxi Provincial Maternal and Child Health Hospital, Nanchang, Jiangxi, China |
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| Abstract: | AbstractOxidative stress (OS) has been proposed to play a role in the development of EMs. Peroxiredoxins are a family of antioxidant proteins that exhibit peroxidase activity in a thioredoxin-dependent manner, protecting cells against OS. The Western blotting results showed that the relative expression of PRDX4 was significantly increased in ectopic endometria compared with the normal endometria of EMs-free (p?2O2 concentration was also significantly higher in the ectopic endometrium. PRDX4 siRNA was transfected into primary ectopic endometrial stromal cells (EESCs). The viability of the transfected EESCs was measured by CCK-8 assay, and the results showed significantly decreased cell viability. Furthermore, the apoptosis rate and ROS generation in flow cytometry assays were significantly increased after the knockdown of PRDX4 expression (p?
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| Keywords: | Endometrial stromal cells peroxiredoxins 4 ROS proliferation invasion |
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