凋亡抑制因子Survivin在肝细胞肝癌的表达及临床意义

目的:探讨Survivin蛋白表达与肝细胞肝癌(HCC)临床病理特征相关性及临床意义.方法:应用链霉菌抗生物素-过氧化物酶免疫组织化学法(SP法),检测67例HCC中Survivin、血管内皮生长因子(VEGF)的蛋白表达.结果:67例肿瘤组织中35例Survivin呈阳性表达,阳性率为52.2%.肿瘤组织表达阳性率高于癌旁组织(P＜0.05).Survivin的表达与HCC肿瘤镜下门静脉癌栓发生密切相关(P＜0.05).VEGF阳性表达者为41例,阳性率为61.1%.VEGF的表达与HCC肿瘤大小、门静脉癌栓发生以及肿瘤TNM分期密切相关(P＜0.05).应用Kaplan-Meier法制作累积生存曲线并经Long-rank检验,结果显示Survivin表达阳性与阴性组患者术后累积生存率有显著性差异(P=0.0018).结论:HCC中Survivin、VEGF蛋白表达上调,Survivin和VEGF均与HCC门静脉癌栓发生密切相关.HCC患者Survivin表达者预后不良,应密切随访.

The Expression of Survivin and Clinical Significance for Patients with Hepatocellular Cancer

Objective: To study the expression of Survivin and the clinical significance for patients with HCC. Methods: Expression of Survivin and VEGF in 67 HCC patients were assessed by immunohistochemistry staining. Results: The Survivin's expression was detected in 35/67 (52.2%). The expression rate of Survivin was higher in HCC group than that of the adjacent nontumorous liver and the normal liver groups (P<0.05). There was significant correlation between the expression of Survivin in HCC and the tumor thrombus in portal vein (P<0.05). VEGF expression rate was 61.1%. The positive rate of VEGF was correlated significantly with tumor size, portal vein tumor thrombus and TNM stage (P<0.05). By Kaplan-Meier Log-rank estimation, it was found the positive expression of Survivin was associated with poorer survival compared with the negative expression(P=0.001 8). Conclusions: Survivin and VEGF is up-regulated in HCC patients. The intrahepatic hematogenous metastasis is related to expression of Survivin and VEGF. Survivin is demonstrated as the prognostic factor for patients undergoing hepatic resection.