| 固相萃取-HPLC法测定人血浆中双氯芬酸钠的浓度 |
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| 引用本文： | 阳利龙,祝文兵,张毕奎,朱运贵,刘晓磊. 固相萃取-HPLC法测定人血浆中双氯芬酸钠的浓度[J]. 中国药房, 2007, 18(8): 594-596 |
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| 作者姓名： | 阳利龙 祝文兵 张毕奎 朱运贵 刘晓磊 |
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| 作者单位： | 1. 湖南省儿童医院药剂科临床药学研究室,长沙市,410007
2. 中南大学湘雅二医院临床药学研究室,长沙市,410011 |
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| 摘 要： | 目的:建立以固相萃取-HPLC法测定人血浆中双氯芬酸钠浓度的方法。方法:色谱柱为ODS-2,流动相为磷酸盐缓冲液(pH6.5)-甲醇(40∶60),检测波长为280nm,流速为1.0mL.min-1。结果:双氯芬酸钠血浆浓度在16.2~2 024.0ng.mL-1范围内与峰面积积分值线性关系良好(r=0.998 9),方法回收率在90%~110%之间,萃取回收率大于90%;日内、日间相对标准差小于5%。结论:本方法操作简便,结果准确可靠,可用于双氯芬酸钠药动学研究。
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| 关 键 词： | 双氯芬酸钠 固相萃取-HPLC法 血药浓度 |
| 文章编号： | 1001-0408(2007)08-0594-03 |
| 收稿时间： | 2006-04-07 |
| 修稿时间： | 2006-06-09 |
Determination of Plasma Diciofenac Sodium by SPE- HPLC |
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| YANG Lilong,ZHU Wenbing,ZHANG Bikui,ZHU Yungui,LIU Xiaolei. Determination of Plasma Diciofenac Sodium by SPE- HPLC[J]. China Pharmacy, 2007, 18(8): 594-596 |
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| Authors: | YANG Lilong ZHU Wenbing ZHANG Bikui ZHU Yungui LIU Xiaolei |
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| Abstract: | OBJECTIVE: To establish an SPE(solid phase extraction)-HPLC method for the determination of diclofenac sodium in human plasma.METHODS: The separation of diclofenac sodium was performed on ODS-2 column with a detection wavelength of 280nm.The mobile phase was composed of phosphate buffer(pH6.5)-methanol(40∶ 60) at a flow rate of 1.0mL· min-1.RESULTS: The linear range of diclofenac sodium was 16.2～ 2 024.0ng· mL-1(r=0.998 9).The methodological recovery ranged between 90% to 110% and the extraction recovery was above 90%.Both intraday RSD and inter-day RSD were less than 5%.CONCLUSION: The method is simple,accurate and reliable,and suitable for the pharmacokinetic study of diclofenac sodium. |
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| Keywords: | Diclofenac sodium SPE-HPLC Blood concentration |
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