空肠弯曲菌HSP43诱导自身免疫机理──分子模拟和抗原优势

我们曾发现空肠弯曲菌４３ｋＤ热休克蛋白（ＨＳＰ４３）能诱导小鼠产生自身免疫应答，本文则进一步分析了这种诱导作用的机理。二株针对ＨＳＰ６０保守区序列的单克隆抗体ＩＩＨ９和ＭＬ－３０不但能结合人及小鼠细胞中ＨＳＰ６０家族成员，而且也能结合ＨＳＰ４３，表明ＨＳＰ４３、人及小鼠ＨＳＰ６０三者均属同一家族成员，具有序列上的高度同源性。小鼠用空肠弯曲菌免疫后出现了针对１０种菌体蛋白的抗体，其中最早被诱导的抗体是针对ＨＳＰ４３的，并且该种抗体在随后８０ｄ观察期间内保持了较高活性，表明ＨＳＰ４３是优势抗原。本文结果提示，ＨＳＰ４３较易被免疫系统识别而产生应答，通过和宿主ＨＳＰ６０高度序列同源性而可能呈现分子模拟，从而诱导自身免疫损伤。

Mechanisms by which the HSP43 Produced by Campylobacter Jejuni Induced Autoimmunity:Molecular Mimicry and Dominant Antigenicity

The mechanism by which 43kD heat shock protein(HSP43)of Campylobacter jejuni induced autoimmunity in mice were studied.Monoclonal antibodies IIH9 and ML-30 against evolutionarily conserved amino acids sequences of HSP60 family recognized not only the members of HSP60 family in human and mice cells but also HSP43 of C.jejuni,suggesting that HSP43 and HSP60 members of human or mice showed high homology in sequences.Serum antibody against HSP43 with highest titre appeared earliest in mice immunized with C.jejuni cells,although other nine proteins of C.jejuni also could induce antibodies production,indicating that HSP43 was a dominant antigen. These results revealed that HSP43 was easily recognized by immune system to develop highly cross-reactivity and the molecular mimicry between HSP43 and members of HSP60 family in hosts.