干扰素及异体骨髓移植对慢性粒细胞白血病细胞β2整合素及L—选择素表达的

目的 探讨慢性粒细胞白血病（ｃｈｒｏｎｉｃ ｍｙｅｌｏｃｙｔｉｃ ｌｅｕｋｅｍｉａ,ＣＭＬ）细胞黏附分子Ｌ－ｓｅｌｅｃｔｉｎ（ＣＤ６２Ｌ）、Ｍａｃ－１（ＣＤ１１ｂ）、ＬＦＡ－１（ＣＤ１１ａ）的表达与ＣＭＬ的发生、疗效的关系。方法 采用三色流式细胞仪检测３４例ＣＭＬ骨髓ＣＤ３４＾＋ＣＤ３８＾－－＋细胞表达ＬＦＡ－１、Ｍａｃ－１、Ｌ－ｓｅｌｅｃｔｉｎ的表达。结果 初治的ＣＭＬ３４＾＋ＣＤ３８＾－－＋细胞黏附分子Ｌ－ｓｅｌｅｃｔｉｎ、ＬＦＡ－１明显低于正常造血细胞的表达。Ｌ－ｓｅｌｅｃｔｉｎ的表达与Ｐｈ’阳性细胞数呈负相关（ｒ＝－０．６８）。８例ＣＭＬ经干扰素治疗后,其Ｌ－ｓｅｌｅｃｔｉｎ、Ｍａｃ－１、ＬＦＡ－１的表达均正常。结论 ＣＭＬＣＤ３４阳性细胞黏附分子Ｌ－ｓｅｌｅｃｔｉｎ、ＬＦＡ－１的低表达反映了ＣＭＬ细胞

Expression of beta 2 integrins and L-selectin on CML cells after treatment with IFN-alpha and allo-bone marrow transplantation

OBJECTIVE: To study the expression of L-selectin, Mac-1, LFA-1 on CML progenitor cells in relation to CML progression and therapeutic effect. METHODS: The expression of adhesion molecules (LFA-1, Mac-1, L-selectin) on bone marrow CD34+ cells from 34 CML patients were analyzed by three-color flow cytometry. RESULTS: The mean percentage of expression of L-selectin, and LFA-1 on CD34+ CD38-(-)+ cells from untreated CML patients was significantly lower than that from normal controls. Among 8 CML patients treated with IFN-alpha, the expression of L-selectin and LFA-1 on CD34+ CD38- cell (37.6 +/- 5.3%, 42.1 +/- 13.1%) was comparable to that from normal controls (38.2 +/- 9.4%, 48.2 +/- 12.2%). L-selectin expression in CD34+ CD38- cells from CML patients was inversely correlated with the percentage of Ph'(+) cells. In 2 CML patients treated with allo-bone marrow transplantation, the expression rate of L-selectin, IFA-1 and Mac-1 on CD38+ CD38- cells was comparable to that from normal controls. CONCLUSION: The data suggest that decreased expression of L-selectin and LFA-1 in CML CD34+ cells reflects one of the features of malignant CML progenitors. IFN-alpha and allo-BMT restore the expression of Mac-1, L-selectin and LFA-1 to normal on CML CD34+ cells.