MGCT与TCT法对新生儿溶血病实验室诊断的分析

目的 :介绍一种新的微柱凝胶抗球蛋白检测技术诊断新生儿溶血病。方法 :采用微柱凝胶技术 (MGCT)和试管抗球蛋白实验 (TCT)同时平行做三项实验进行对比 :①直接抗球蛋白试验 ,②游离IgG抗体试验 ,③放散试验。结果 :MGCT与TCT两种方法检测 98例 (均为Rh阳性 ) ,疑为新生儿溶血病的样本 ,阳性率分别为 6 5 .3% (6 4例 ) ;4 9.9% (4 6例 )。两种方法检测HDN的发生呈显著差异 ,具有统计学意义 (P <0 .0 5 )。结论 :微柱凝胶技术具有敏感性高、操作简便、受人为因素影响少和结果可保存等优点 ,不足之处是价格偏高

Diagnosis of Hemolytic Disease of Newborn with MGCT and TCT

Objective To introduce a new micro column gel Coombs test to diagnosis of hemolytic disease of newborn. Methods Three parallel tests were performed with micro column gel test and traditional tube-based Coombs test:①to assay direct anti-human globulin in 98 cases with micro column gel Coombs test, ②free IgG antibody test, ③elution test. Results: 98 cases (all were Rh(+)) suspected of hemolytic disease of newborn were assayed by MGCT and TCT. Positive rates were 65.3 % and 49.9 % , respectively. Significantly statistical difference was found between the positive rates (P<0.05 ) . Conclusion The micro column gel technology has the advantages of high sensitivity, simple operation, preservable results and less influence by artificial factors, and its disadvantage is high price.