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| 椎茸的乙酸乙脂提取物诱导人乳腺癌MCF-7细胞凋亡的作用 | |
| 引用本文： | 李庆林,骆宏丰,方念白.椎茸的乙酸乙脂提取物诱导人乳腺癌MCF-7细胞凋亡的作用[J].中国药理学通报,2006,22(8):1001-1004. |
| 作者姓名： | 李庆林 骆宏丰 方念白 |
| 作者单位： | 1. 安徽中医学院安徽省现代中药重点实验室,安徽,合肥,230038 2. 中国科学院上海药物研究所,上海,201203 3. Arkansas University for Medical Science Little Rock,AR 72202,USA |
| 摘 要： | 目的观察椎茸的乙酸乙脂提取物对体外培养的人乳腺癌MCF7细胞株诱导凋亡的作用。方法MTT法测定提取物对MCF7细胞生长的影响;AnnexinVFITC和PI染色,采用FCM测定MCF7细胞的凋亡指数;采用FCM分析细胞周期的影响;Westernblot法分析CyclinD1、Cdk4、Bax和p21WAE1/CIP1表达。结果椎茸的乙酸乙脂提取物可以剂量依赖性的抑制乳腺癌细胞株MCF7的生长,使MCF7细胞生长停滞在G0/G1期而诱导MCF7细胞凋亡;通过Bax和p21WAE1/CIP1的表达和降低CyclinD1、Cdk4的表达而诱导MCF7细胞的凋亡。结论椎茸的乙酸乙脂提取物通过诱导细胞凋亡而产生抗乳腺癌的活性。 |
| 关 键 词： | 椎茸乙酸乙脂提取物 MCF-7 细胞凋亡 细胞周期 |
| 文章编号： | 1001-1978（2006）08-1001-04 |
| 收稿时间： | 2006-04-27 |
| 修稿时间： | 2006-06-10 |
Ethyl acetate extracted from Shiitake Mushroom induced apoptosis in a breast cancer cell line-MCF-7 |
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| LI Qing-lin,LUO Hong-feng,FANG Nian-bai.Ethyl acetate extracted from Shiitake Mushroom induced apoptosis in a breast cancer cell line-MCF-7[J].Chinese Pharmacological Bulletin,2006,22(8):1001-1004. | |
| Authors: | LI Qing-lin LUO Hong-feng FANG Nian-bai |
| Institution: | 1. Anhui College of Traditional Chinese Medicine ,Anhui key Laboratory of Modernized Chinese Material Medica, Hefei 230038, China ;2. Shanghai Institute of Material Medica, Chinese Academy of Sciences, Shanghai 201203 ,China;3. Arkansas University for Medical Science, Little Rock,AR 72202 USA |
| Abstract: | Aim This study was to examine the effect of ethyl acetate extract of shiitake mushroom on a human breast cancer line-MCF-7 cell in vitro. Method Cell cytotoxicity was examined using MTT method, Cell apoptosis was examined by AnnexinV-FITC/PI staining,Cell cycle analysis was determined by flow cytometry. Western blot analysis was used to determine Cyclin D_1、Cdk4、Bax and p21WAE1/CIP1 protein expression. Results Concentration-dependent cytotoxic effects of the extract were observed in MCF-7 human breast cancer cell lines. Pro-apoptotic proteins (Bax, p21WAE1/CIP1)were up-regulated while proteins involved in cell cycle(Cyclin D_1 and Cdk4)were down-regulated. Conclusion This study suggests that the anti-breast cancer activities might result from increased apoptosis and growth arrest |
| Keywords: | MCF-7 |
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