乙肝患者HBV前S1抗原与HBV-DNA相关性探讨

目的:探讨乙肝病毒(HBV)前S1抗原(Pre-S1Ag)与e抗原(HBeAg)、HBV-DNA含量的相关性,以评价Pre-S1Ag检测在乙型肝炎诊疗中的价值.方法:临床采集乙肝表面抗原阳性标本315例,以ELISA法检测Pre-S1Ag和HBeAg,实时荧光定量PCR(FQ-PCR)法检测HBV-DNA含量,并分析P...

Correlation between Pre-S1 Ag and HBV-DNA of patients with hepatitis B

Objective： To explore the relationship among Pre-S1Ag,HBeAg and HBV-DNA of hepatitis B virus（HBV）,and to evaluate the application of Pre-S1 in diagnosis and treatment.Methods： A total of 315 cases with positive HBsAg were selected.Pre-S1 Ag,and HBeAg were determined by ELISA,while the level of HBV-DNA was measured by RT-PCR.And the relationship among Pre-S1Ag,HBeAg and HBV-DNA was analyzed.Results： Pre-S1Ag was associated with HBeAg and HBV-DNA（χ2=85.2 and 159.22,respectively,P〈0.01）. The positive rate of Pre SlAg was 37.0%, and the positive rate of HBV-DNA was 43.2% in HBeAg negative group; while in HBV-DNA positive patients （≥500 copies/ mid, 84.20..4o had the positive rate of Pre-S1Ag, 52.6% had the positive rate of HBeAg. There was signif- icant difference between two groups （P〈0. 01）, and the positive rate of Pre-S1Ag increased as HBV- DNA. The sensitivity, accuracy, and odds ratio of Pre-SIAg were higher than those of HBeAg （4.2% VS 52.6%, 85.7% VS 69.2%, 39.1 VS 18.7）; while specify, positive predictive value and positive likelihood ratio were similar （88.0%VS 94.4% ,91.4% VS 93.40%,7.01 VS 9.39）. Conclusions： Pre-S1Ag is more sensitive and accurate in reflecting the duplication of HBV, and has higher correlativity with HBV DNA. It is valuable in diagnosis and treatment of hepatitis B.