阿仑膦酸钠防治牙槽骨吸收的实验研究

目的用骨质疏松及牙槽骨吸收动物模型评价阿仑膦酸盐对骨吸收的防治作用。方法建立骨质疏松及牙槽骨吸收的动物模型，建模手术次日，给实验组皮下注射阿仑膦酸钠液，每周3次，共6周。用血生化指标、骨生物力学、骨密度测量及组织形态学方法进行药效评价。结果股骨密度对照组（0.123g/cm2）与实验组（0.151g/cm2）相比明显降低（P<0.01）；血清碱性磷酸酶对照组（259.63U/L）与实验组（151.13U/L）相比明显升高(P<0.01)；骨生物力学各项指标，实验组与对照组也有明显差异。组织形态学实验组牙龈轻度炎症，牙槽嵴顶未见明显吸收；对照组牙龈乳突炎症明显，上皮钉突增生，炎细胞浸润，牙槽嵴顶明显吸收。结论阿仑膦酸钠能减少骨质丢失从而可以防止骨质疏松及病理性牙槽骨骨吸收。

The treatment of osteoporosis and bone resorption of alveoli with alendronate in rat model

Objective To evaluate the anti osteoporosis effect of alendronate on bone in rat model. Methods The osteoporosis and alveolar bone resorption animal model were established in rats. The experimental groups were given alendronate in 5 mg/kg BW by subcutaneous injection after first day of ovariectomy and dental ligature, three times a week for 6 weeks. All the animals were sacrifised 12 weeks after the operation. The blood samples were collected for determination of biochemical indices. The left femora and jaw bones were processed for histomorphometry. The right femora and mendibles were prepared for determination of bone density and bone biodynamics. Results As compared with the control groups(without alendronate), the values of experimental groups (with alendronate) were all significantly improved on bone density, bone anti flexure ability and body weight. The alkaline phosphatase, calcium in serum of ovariectomy groups(without alendronate) were obviously increased. In the experimental group, however, these indices were close to normal levels. In histomorphometry, there were little inflammation in gingiva and no obvious bone resorption in the alveolar crests of the experimental groups; but there were obvious gingivitis and alveolar crests resorption of the control groups. Conclusions Alendronate can effectively prevent bone loss to reduce osteoporosis in ovariectomized rats and prohibit pathological alveolar bone resorption.