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| 青南高原地区高原鼠兔棘球蚴的分子鉴定和遗传分化分析 | |
| 引用本文： | 马俊英,林恭华,王虎,吴献洪,贾宁. 青南高原地区高原鼠兔棘球蚴的分子鉴定和遗传分化分析[J]. 寄生虫与医学昆虫学报, 2011, 18(3): 147-151. DOI: 10.3969/j.issn.1005-0507.2011.03.004 |
| 作者姓名： | 马俊英 林恭华 王虎 吴献洪 贾宁 |
| 作者单位： | 1. 青海省地方病预防控制所,西宁,811602 2. 中国科学院西北高原生物研究所,西宁,810008 3. 甘肃农业大学动物医学院,兰州,730070 |
| 摘 要： | 基于线粒体cox1基因部分序列对青南高原地区称多、达日、玛沁3县高原鼠兔感染的20份棘球绦虫幼虫(棘球蚴)样品进行分子鉴定和遗传分化分析.在测序获得的700 bp长度序列中检测到17个变异位点,其中11个为简约信息位点.共检测到9个单倍型,最大简约法和最大似然法都表明这些单倍型都属于石渠棘球绦虫.遗传距离和聚类分析显示... |
| 关 键 词： | 石渠棘球绦虫 分子鉴定 遗传分化 谱系关系 |
Molecular Identification and Genetic Variations of Echinococcus in Plateau Pika from Eastern and Southern Qinghai, China |
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| MA Jun-Ying,LIN Gong-Hua,WANG Hu,WU Xian-Hong,JIA Ning. Molecular Identification and Genetic Variations of Echinococcus in Plateau Pika from Eastern and Southern Qinghai, China[J]. Acta Parasitologica et Medica Entomologica Sinica, 2011, 18(3): 147-151. DOI: 10.3969/j.issn.1005-0507.2011.03.004 | |
| Authors: | MA Jun-Ying LIN Gong-Hua WANG Hu WU Xian-Hong JIA Ning |
| Affiliation: | MA Jun-Ying LIN Gong-Hua WANG Hu. WU Xian-HongI JIA Ning3. (1. Qinghai Institute for Endemic Disease Prevention and Control, Qinghai 811602, China; 2. Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Qinghai 810008, China ; 3. College of Veterinary Medicine, Gansu Agriculture University, Lanzhou 730070, Gansu, China) |
| Abstract: | Based on mitochondrial coxl gene, we analyzed the taxonomic status and genetic variations of 20 Echinococcus samples in plateau pika from Chengduo （CD） , Dari （DR） and Maqin （MQ） in eastern and southern Qinghai, China. From the sequenced 700 bp sequences, we detected 17 variable sites, in which 11 were parsimony informative sites. We identified nine haplotypes, all of which were E. shiquicus based on maximum parsimony and maximum likelihood analyses. CD and DR had a closer relationship than among other populations, while MQ had a far phylogenetic relationship from CD, DR and Shiqu （SQ, where E. shiquicus was first reported）; moreover, MQ had higher genetic diversities than CD and DR, suggesting that MQ might be one of the origins in the eastern and southern Qinghai region. The statistical parsimony networks of E. shiqtticus haplotypes showed a star-like expansion from a main founder haplotype, which was different from the former study showing a divergent network, indicating that the E. shiquicus might have a more complex historic genetic variation process than expected. |
| Keywords: | Echinococcus shiquicus Molecular identification Genetic variation Haplotype network |
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