| 南宁地区人群血小板抗原HPA1—16bw基因分布频率的研究 |
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| 引用本文： | 廖燕,申卫东,李丽兰,李恒聪,李彬,吴国光.南宁地区人群血小板抗原HPA1—16bw基因分布频率的研究[J].中国输血杂志,2010,23(6). |
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| 作者姓名： | 廖燕 申卫东 李丽兰 李恒聪 李彬 吴国光 |
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| 作者单位： | 南宁市中心血站,南宁输血医学研究所,广西,南宁,530003 |
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| 摘 要： | 目的研究南宁地区人类血小板抗原基因分布频率,为临床同种免疫血小板减少症患者提供诊断依据和HPA相容的成分血。方法采用序列特异性引物聚合酶链反应(PCR-SSP)对南宁地区1 500名无血缘关系的成年人做HPA1—16bw基因分型研究。在包含引物混合液的96孔反应板中,按照相同的循环条件扩增PCR产物。结果每份血液标本均可在4 h内获得分型结果。在16个HPA系统中,HPA-15基因型杂合频率最高,HPA15a/15a,HPA15a/15b和HPA15b/15b的频率分别为0.252 7,0.500 7和0.246 7。HPA-3的杂合程度仅次之,HPA3a/3a,HPA3a/3b和HPA3b/3b的频率分别为0.284 0,0.508 0和0.208 0。其余14个HPA系统均以a/a纯合子为主,其a基因频率范围为0.916 7—0.999 3。除了HPA-3b/3b和HPA-15b/15b的纯合子以外,还检测出了HPA-2b/2b5例和HPA-1b/1b1例。HPA-7bw至-14bw,-16bw的a/a等位基因纯合率为1.000 0。结论在临床工作中我们仍须警惕可能由HPA-1,-2,-3,-5,-6和-15同种抗体引起的疾病,HPA基因分型将有助于更好地诊断同种免疫血小板减少症和建立相容的HPA血小板供者库,提供更有效的血小板输注方法。
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| 关 键 词： | 人类血小板同种抗原(HPA) PCR-SSP 基因分型 南宁人群 |
Genetic frequency distribution of human platelet antigens 1-16bw in Nanning population in China |
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| LIAO Yan,SHEN Weidong,LI Lilan,LI Hengcong,LI Bin,WU Guoguang.Genetic frequency distribution of human platelet antigens 1-16bw in Nanning population in China[J].Chinese Journal of Blood Transfusion,2010,23(6). |
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| Authors: | LIAO Yan SHEN Weidong LI Lilan LI Hengcong LI Bin WU Guoguang |
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| Institution: | LIAO Yan,SHEN Weidong,LI Lilan,et al.Corresponding author:WU Guoguang. Nanning Institute of Transfusion Medicine,Nanning Blood Center,Nanning 530003,China |
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| Abstract: | Objective To study the genetic frequency distribution of human platelet antigens in Nanning area,and to provide diagnosis and HPA-matched blood components for patients with alloimmune thrombocytopetic syndromes.Methods A total of 1 500 blood samples from unrelated adults in Nanning were included in the study.Polymerase chain reaction with sequence-specific primers(PCR-SSP) was employed for simultaneous HPA-1 to HPA-16bw genotyping.All PCR amplifications were carried out under identical thermo-cycle conditio... |
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| Keywords: | PCR-SSP |
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