乙酰肝素酶、nm23-H1在鼻咽癌中的表达以及与侵袭转移的关系

目的 探讨乙酰肝素酶(HPA)和nm23-H1在鼻咽癌组织中的表达以及与肿瘤侵袭转移的关系。方法 用免疫组化技术检测HPA和nm23 H1在60例鼻咽癌组织和20例鼻咽慢性炎症组织中的表达情况，并结合患者的临床病理指标进行分析。结果 HPA在鼻咽慢性炎症组织和鼻咽癌组织中的阳性表达率分别为0（0/20）和55%（33/60）（P＜0.05）；nm23 H1在鼻咽慢性炎症组织和鼻咽癌组织中的阳性表达率分别为90.0%（18/20）和53.3%（32/60）（P＜0.05）。在鼻咽癌组织中，随着鼻咽癌临床分期和侵袭程度的升级，以及有颈淋巴结转移和远处转移时，HPA阳性表达逐渐增加，而nm23-H1阳性表达逐渐减少（P<0.05）；HPA和nm23-H1的表达呈明显负相关（P＜0.05）。结论 HPA的阳性表达和nm23-H1阴性表达的鼻咽癌患者有较高的侵袭转移潜能。

Expressions of heparanase and nm23-H1 protein and their relationship with invasion and metastasis in nasopharyngeal carcinoma

Objective To investigate expressions of heparanase and nm23-H1 protein in nasopharyngeal carcinoma (NPC) as well as their relationship with clinicopathological factors. Methods Expressions of heparanase and nm23-H1 protein in 60 cases of NPC with poor differentiation and 20 cases of chronically inflammatory tissues of nasopharynx were determined by immunohistochemical staining. Results The positive rate of heparanase protein in tumor tissues was 55% (33/60), which was significantly higher than that in inflammatory tissues (P<0.05). The heparanase protein expression was significantly related to TNM stage, local lymph nodes and distant metastasis of NPC (P<0.05). The positive rate of nm23-H1 protein in tumour tissues was 53.3% (32/60), significantly lower than that in inflammatory tissues (P<0.05), and the nm23-H1 protein expression was significantly related to clinical stage, local lymph nodes and distant metastasis of NPC(P<0.05). Expression of heparanase protein had a significantly negative correlation with that of nm23-H1 protein in NPC. Conclusion The positive expression of heparanase and the negative expression of nm23-H1 may be significantly related to the invasive infiltration and metastasis in NPC.