抗癌药物诱导人B淋巴细胞白血病细胞的凋亡及周期特异性

目的探讨抗癌药物对人Ｂ淋巴细胞白血病细胞株Ｎａｌｍ－６细胞凋亡（ａｐｏｐｔｏｓｉｓ）的影响及周期特异性。方法临床常用的抗癌药物与Ｎａｌｍ－６细胞相互作用８～２４小时后,利用低倍体ＤＮＡ－ＦＣＭ测定法和ＴｄＴ测定＋ＤＮＡ染色法进行检测。结果ＡＤＲ、ＶＰ１６、ＣＰＴ、ＭＴＸ和Ａｒａ－Ｃ能明显诱导细胞凋亡,主要作用于Ｓ期。ＣＰＭ、６ＭＰ和ＰＲＤ则有较低的凋亡细胞诱导率,ＣＰＭ在大剂量时主要引起细胞坏死。６ＭＰ诱导Ｇ１和Ｓ期细胞凋亡,ＰＲＤ诱导Ｇ１期细胞凋亡,ＣＰＭ诱导凋亡作用无明显的周期特异性。结论抗癌药物能诱导人Ｂ淋巴白血病细胞株Ｎａｌｍ－６细胞凋亡。低倍体ＤＮＡ－ＦＣＭ测定法能检测细胞的凋亡率,ＴｄＴ测定＋ＤＮＡ染色法能呈现凋亡细胞与细胞周期的关系。临床上可利用此两种方法检测肿瘤细胞的凋亡率,抗癌药物的疗效及其周期特异性。从而有助于化疗方案的设计及预后的评估。

Apoptosis of B lymphocytic leukemia induced by anticancer drugs and their cell cycle specificity

OBJECTIVE: Evaluating the effect of apoptosis induced by anticancer drugs on human B lymphocytic leukemia cell lines (Nalm-6) and their cell cycle specificity. METHODS: Nalm-6 cells were treated with various anticancer drugs for 8-24 hours. Apoptotic cells and their cell cycle specificity were measured by using hypodiploid DNA-FCM and TdT assay + DNA staining. RESULTS: ADR, VP16, CPT, MTX, Ara-C could markedly induce apoptosis of Nalm-6 cells in S phase. CPM, PRD, 6MP were less capable of inducing apoptosis. CPM in high dose resulted in cell necrosis. PRD induced apoptosis in G1 phase, while 6MP induced apoptosis in G1 and S phase. The effect of CPM showed no marked cell cycle specificity. CONCLUSION: Hypodiploid DNA-FCM and TdT assay + DNA staining can be used to detect both tumor cell apoptosis and their cell cycle specificity which is helpful to predict prognosis and to design new chemotherapy regimen.