酶联免疫印迹术（EITB）在小鼠日本血吸虫感染早期诊断中的应用

为探讨日本血吸虫感染的早期诊断方法,本文应用酶联免疫印迹术(EITB)检测感染鼠血清的循环抗原。所用抗体由日本血吸虫成虫和虫卵的五种抗原(SEA、UEA、TA、CY、MC)免疫动物获得。SDS—PAGE和EITB显示这些抗原与华支睾吸虫、肺吸虫和蛔虫等交叉反应不明显。其中CY抗原的31KD条带是血吸虫特有。用EITB监测小鼠感染日本血吸虫后6周内各期和用吡喹酮治疗后2周的循环抗原有如下变化:UEA抗体显示的187KD抗原多肽在4周出现并逐渐加深,治疗后变淡;与TA和CY抗血清反应的40KD抗原分别在感染后3天～4周和3天～2周出现;抗MC显示的15.3KD在6周出现,治疗后消失;与成虫表膜相关的20、15、12.8KD仅在治疗后出现。对这些特异的抗原性多肽动态观察似可为早期诊断日本血吸虫病和考核疗效提供参考。

Application of EITB to the Early Diagnosis of Experimental Schistosoma japarucum in Mice

EITB was used to detect the circulating antigens of Schistusoma japonicum (S. j)in the sera of infected mice. The antibodies used were prepared from 5 antigens (SEA, UEA, TA, CY , MC) which, showed by SDS-PAGE and EITB, appeared specific to some other helminthes. The 31 KD peptide of CY antigen was only possessed by Schistosome. There were some characteristic changes of circulating antigens within the sera of mice after infected with S. j and treated by praziquantel: 187 KD peptide of UEA appeared from 4 weeks after infection and became lighter 2 weeks after treatment. 40 KD peptide reacting to anti-TA and anti-CY antibodies was demonstrated from the third day to the fourth week and from the third day to the second week respectively. 15. 3 KD detected by anti-MC appeared 6 weeks after infection and disappeared after treatment. Some antigens relating to the tegument of adult like 20, 15, 12. 8 KD were demonstrated only after treatment. It possibly proved that detection of antigenic peptides as mentioned above in infected animals may give a testimony to early diagnosis and assessment of curing in schistosomiasis.