小型猪颈动脉粥样硬化性狭窄模型的建立

目的：建立小型猪颈动脉粥样硬化性狭窄模型。方法：采用过大球囊扩张小型猪一侧颈总动脉后高脂饲养3个月。分别在球囊扩张前、扩张后2、4、8、12周末，取静脉血检测血脂水平；用彩色超声多普勒观察颈动脉粥样硬化及狭窄程度；第12周末处死动物，观察双侧颈总动脉的病理改变。结果：超声显示球囊扩张侧均出现不同程度的动脉粥样硬化并管腔狭窄：病理显示损伤侧颈总动脉明显粥样斑块形成，血管壁各层改变符合动脉粥样硬化特点。结论：通过过大球囊损伤加高脂饲养方法可成功建立小型猪颈动脉粥样硬化狭窄的动物模型。

Construction of carotid artery atherosclerotic stenosis model in mini-swine

Objective: To establish carotid artery atherosclerotic stenosis model in mini-swine. Methods: Mini-swines were fed with high lipid diet for 3 months after dilataltion of unilateral carotid artery by larger sacculus. The serum levels of triglyceride and cholesterol were examined before and at the end of the 2 nd, 4 th, 8 th, 12 th week after operation. The formation of atherosclerosis and stenosis in the injuried artery were observed by Doppler ultrasound. Then the animals were killed at the end of 12 th week and observed the pathologic changes in bilateral carotid arteries. Results: The ultrasound showed the formation of atherosclerosis and stenosis in the injuried arteries. The incrassated intima and the atheromatous plaques accorded with the atherosclerosis in human. Conclusion: Injuring the intima by dilatalting the carotid artery with larger sacculus plus high lipid diet can successfully establish carotid artery atherosclerotic stenosis model in mini-swine.