标本放置时间对尿液中红细胞、白细胞测定的影响

目的探讨不同放置时间对尿液细胞计数及红细胞形态的影响。方法用UF-1000i全自动尿沉渣分析仪测定即刻、1、2、3、4、5 h各时间点尿液红细胞、白细胞、上皮细胞的数量,并用相差显微镜观察各时间点尿液中红细胞的形态变化。结果与即刻测定结果比较尿红细胞计数随放置时间延长呈趋势性降低,并在4 h后降低差异有统计学意义（P〈0.05）,白细胞和上皮细胞计数5h内结果稳定,差异无统计学意义（P〉0.05）,小红细胞5 h内形态未发生显著变化,大红细胞在3 h后出现皱缩、异常红细胞增多等形态的改变。结论为确保尿液常规检验结果,尤其是尿红细胞形态的准确可靠,标本必须新鲜,并尽可能在4 h内完成检测。

The influence of urine specimen storage time on the determination results of RBC and WBC in urine

Objective To investigate the influence of different urine specimen storage time on the count of the cells in urine and the morphology of red blood cell.Methods The automatic urinary sediment analyzer UF-1000i was used to analyze the numbers of red blood cell,white blood cell and epithelial cell in urine immediately and after 1 h,2 h,3 h,4 h and 5 h.The morphology of red blood cell was observed by microscopy simultaneously.Results Compared with the results of fresh urine,the number of red blood cell had an obvious decreasing change after 4 h（P0.05）.The numbers of white blood cell and epithelial cell did not show obvious change within 5 h（P0.05）.The morphology of microcyte did not alter within 5 h,while the morphology of the macrocyte had the changes of crenulation and abnormal red blood cell increasing after 3 h.Conclusions For ensuring a reliable and accurate results,specially the morphology of red blood cell in urine,the urine specimen should be fresh and be determined within 4 h.