| 鞘内注射可乐定对神经病理性疼痛大鼠背根神经节内交感神经芽生的影响 |
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| 引用本文： | 王海涛,杨美蓉,颜涛,李士通.鞘内注射可乐定对神经病理性疼痛大鼠背根神经节内交感神经芽生的影响[J].上海医学,2011,34(2). |
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| 作者姓名： | 王海涛 杨美蓉 颜涛 李士通 |
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| 作者单位： | 上海交通大学附属第一人民医院麻醉科,200080 |
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| 摘 要： | 目的观察鞘内注射可乐定对神经病理性疼痛(NPP)大鼠痛阈及对背根神经节(DRG)内篮状结构数量的影响.方法通过左侧L5脊神经结扎(SNL)建立NPP模型.32只雄性Sprague-Dawley大鼠鞘内留置给药导管后随机分为4组:假手术伍用0.9%氯化钠溶液组(S组),假手术伍用可乐定组(SC组),SNL伍用0.9%氯化钠溶液组(L组),SNL伍用可乐定组(LC组).术前测量大鼠机械性痛阈50%缩爪阈值(50%PWT)]基础值;术后7 d内,每天鞘内注射可乐定或0.9%氯化钠溶液,1 h后测量各组大鼠的50%PWT.术后第8天,取各组大鼠左侧L5、L4及右侧L5 DRG,通过免疫组织化学法观察DRG内篮状结构数量的变化.结果术后1～7 d,L组大鼠的50%PWT均显著低于S组同时间(P值均<0.05),LC组大鼠的50%PWT均显著高于L组同时间(P值均<0.05),SC组与S组间大鼠的50%PWT的差异均无统计学意义(P值均>0.05).术后第8天,与S组比较,L组术侧L5、L4DRG内篮状结构的数量显著增多(P值均<0.05),对侧L5 DRG内篮状结构的数量略有增加,但差异无统计学意义(P>0.05);与L组比较,LC组术侧L5、L4 DRG内篮状结构的数量显著减少(P值均<0.05);SC组与S组间术侧L5、L4及对侧L5 DRG内篮状结构的数量的差异均无统计学意义(P值均>0.05).结论可乐定可能通过减少DRG内交感神经节后纤维芽生而发挥其对NPP的镇痛作用.
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| 关 键 词： | 可乐定 神经病理性疼痛 背根神经节 交感神经芽生 |
Effects of intrathecal infusion of clonidine on sympathetic sprouting in the dorsal root ganglion of rats after spinal nerve ligation |
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| WANG Haitao,YANG Meirong,YAN Tao,LI Shitong.Effects of intrathecal infusion of clonidine on sympathetic sprouting in the dorsal root ganglion of rats after spinal nerve ligation[J].Shanghai Medical Journal,2011,34(2). |
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| Authors: | WANG Haitao YANG Meirong YAN Tao LI Shitong |
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| Institution: | WANG Haitao,YANG Meirong,YAN Tao,LI Shitong. Department of Anesthesiology,Shanghai First People's Hospital,Shanghai Jiaotong University,Shanghai 200080,China |
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| Abstract: | Objective To investigate the effect of clonidine on pain threshold and basket structures in the dorsal root ganglion(DRG)of rats with neuropathic pain.Methods The neuropathic pain model was developed by left L5 spinal nerves ligation(SNL).Thirty-two male adult Sprague-Dawley rats weighing 220-240 g were randomly divided into four groups after intrathecal catheterization.Group S:sham surgery with saline intrathecal injection;Group SC:sham surgery with clonidine injection;Group L:SNL with saline injection;and... |
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| Keywords: | Clonidine Neuropathic pain Dorsal root ganglia Sympathetic sprouting |
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