钙离子拮抗剂抗大鼠皮瓣脂质过氧化作用的探讨

目的：探讨钙离子桔抗剂对大鼠腹部皮瓣缺血再灌注损伤的保护作用．方法：建立大鼠腹壁下动脉岛状皮瓣缺血再灌注模型，检测应用钙离子桔抗剂维拉帕米（Ｖｅｒａｐａｍｉｌ，Ｖｅｒ）和汉防己甲素（Ｔｅｔｒａｎｄｃｉｎ，Ｔｅｔ）前后，皮瓣脂肪组织中超氧化物歧化酶（ＳＯＤ）活性、谷胱甘肽过氧化酶（ＧＳＨ－Ｐｘ）活性和丙二醛（ＭＤＡ）含量的变化．结果 Ｖｅｒ和 Ｔｅｔ可以明显地提高皮瓣的 ＳＯＤ活性和 ＧＳＨ－Ｐｘ活性，并可显著降低 ＭＤＡ的含量．结论：钙离子桔抗剂能减轻大鼠腹部皮瓣缺血再灌注损伤的脂质过氧化作用．

Study of the Antioxidation Effect of Calcium Antagonist in Rat Abdominal Skin Flap

Objective: To observe the protective effect of calcium antagonist on the ischemic reperfusion injury in rat abdominal skin flap. Methods: 33 SD rats were randomized into three groups. The abdominal island flap based on the inferior epigastric vessels was used as the ischemic reperfusion model. In the control group, normal saline was given intraperitoneally for 3 consecutive days preoperatively, while Verapamil(Ver) and Tetrandcin(Tet) were given in the other two groups respectively. Samples from the flap were harvested during ischemia arid reperfusion periods to measure the changes of superoxide dismutase (SOD) activity, glutathione peroxidase (GSH-Px) activity and malonyl dialdehyde (MDA) content. Results: SOD activity and GSH-Px activity were much higher in the Ver and Tet groups than that in the control group, while the MDA content was remarkably decreased in these two groups. Conclusions: Calcium antagonist, Verapamil and Tetrandcin might play an important role oxygen free radical scavenging and in reducing lipid peroxidation.