妊娠高血压综合征患者胎盘滋养细胞线粒体ATP酶和氧自由基变化的研究

目的 测定妊高征患者胎盘滋养细胞线粒体ATP酶活性及氧自由基的变化，探讨其在妊高征发病机制中的作用。方法 选取妊高征患者（53例）及正常妊娠妇女（32例）胎盘，匀浆后提取线粒体。分别测定线粒体Ca^2＋-Mg^2＋-ATP酶、SOD的活性及MDA含量变化。结果 重度妊高征患者胎盘滋养细胞线粒体Ca^2＋-Mg^2＋-ATP酶、SOD活性与正常孕足月胎盘相比明显降低，而MDA含量却明显升高（P〈0．05）；Ca^2＋-Mg^2＋-ATP酶活性与MDA含量呈负相关，而与SOD呈正相关。结论 重度妊高征患者胎盘滋养细胞线粒体ATP酶活性显著降低，自由基增多，提示自由基抑制ATP酶活性。促进妊高征病情进展。

Study of activity of ATPase and oxygen-derived free radidicals of placenta cytotrophoblasts mitochondrion in patients with pregnancy induced hypertension

Objective] To investigate the role in pathogenesis of pregnancy induced hypertension(PIH).Methods] 53 women with PIH(25 mild or moderate,28 severe PIH)and 32 women with normal pregnancy were recruited as study group and control,respectively.The mitochondria of placenta cytotrophoblasts were estracted and the activity of the enzyme and concentration of malondialdehyde(MDA)were measured.Results] The activity of Ca2+-Mg2+-ATPase and SOD of mitochondria of placenta cytotrophoblasts in severe PIH were lower than control,while the activity of MDA was higher than control(P <0.05).Positive correlation between Ca2+-Mg2+-ATPase and SOD,but negative correlation between Na+-K+-ATPase,Ca2+-Mg2+-ATPase and MDA were found both in study group and control group.Conclusion] Decrease of the activity of ATPase but increase of oxygen-derived free radidicals of placenta cytotrophoblasts mitochondrion in patients with pregnancy induced hypertension suggested that free radical suppressed ATPase and facilitate PIH.