食管癌及大肠癌和癌旁组织端粒酶活性的临床意义

目的：建立测定端粒酶活性的PCR－TRAP方法，并探讨其在食管癌、大肠癌诊断的意义。方法：应用端粒酶活性的PCR－TRAP方法，对36例食管癌、40例大肠癌及其癌旁组织进行了检测。结果：食管癌组织与癌旁组织端粒酶活性阳性检出率相比，差异有非常显性；伴随淋巴结转移的标本中端粒酶阳性检出率与未伴随淋巴结转移的标本比较，具有非常显性差异。40例大肠癌组织中端粒酶活性阳性检出率明显高于癌旁组织（P＜0．001）；伴随淋巴结转移的标本中端粒酶活性阳性检出率与未伴随淋巴结转移的标本相比，差异无显性。结论：端粒酶活性增高可能是致食管、大肠组织癌变的重要因素；端粒酶活性的检出可作为食管癌、大肠癌诊断的指标之一。

Telomerase cctivity in human esophageal cancer and rectal-colon Carcinoma

AIM: To evaluate the diagnostic value of telomerase activity in patients with esophageal cancer and rectal-colon carcinoma. METHODS: Telomerase activity in esophageal cancer and rectal-colon carcinoma and their adjacent tissues in 76 patients was detected by using the PCR-TRAP method. RESULTS: There was significant difference in positive rate of telomerase activity between esophageal cancer and their adjacent tissues; the positive rate of telomerase activity in esophageal cancer with lymph node metastasis was higher than that without lymph node metastasis (P=0.006). In 40 specimens of human rectal-colon carcinoma and their adjacent tissues, the positive rates of telomerase activity were 91.75% and 5.00% respectively (P<0.001). Meanwhile, telomerase activity was not related to the differentiation of malignance and metastasis. CONCLUSION: PCR-TRAP is a valuable method for assaying telomerase activity , which may be a diagnostic marker of esophageal cancer and rectal-colon carcinoma especially in early stage.