树鼩部分信号转导通路的相关cDNA芯片的建立

目的：建立树鼩部分信号转导通路的相关cDNA芯片，从而进一步探讨肝细胞癌（HCC）发生的分子机制。方法：在基因库中找出人的信号转导通路相关基因mRNA序列，选取其中与大、小鼠高度同源的片段，设计出相关的引物序列，然后用树鼩正常肝组织的cDNA为模板经PCR扩增出树鼩相应基因的cDNA片段，将这些cDNA片段作为树鼩的特异性探针，进而建立起树鼩部分信号转导通路的相关cDNA芯片。结果：得到涉及信号转导通路相关基因的探针共有152个基因。结论：利用该芯片研究树鼩HCC发生过程中信号转导通路中的作用将有重要的实用价值，从而完善树鼩肝癌动物模型的研究。

THE ESTABLISHMENT OF cDNA MICROARRAY ABOUT PARTS OF SIGNAL TRANSDUCTION PATHWAY IN TREE SHREWS

Objective:The cell signal transduction has been the focus and in the fore-front of scientific research since the 90s of 20th century.The technique of cDNA microarray has been applied to the study of tumor cell signal transduction.The hepatocellular carcinoma(HCC) model of tree shrew is a useful animal model for studying the initiation and progression of HCC.As yet,there isn't cDNA microarray of signal transduction pathway in tree shrews.The purpose of this study is to establish the cDNA microarray about parts of signal transduction pathway in tree shrews,thereby to further explore the molecular mechanism of HCC occurrence.Methods:The study was done by finding the human relative gene's mRNA sequence in gene bank,and then,selecting the most homologous segment to design the primer,and then to acquire the correlative cDNA segment through PCR enlargement by using the primer and normal liver tissue's cDNA as a template.Special probes were made by using the cDNA segment,and then establishing the cDNA microarray of signal transduction pathway in tree shrews.Result:We have got 152 genes in the cDNA microarray of signal transduction pathway in tree shrews.Conclusion:The establishment of cDNA microarray about parts of signal transduction pathway may be a very important virtual utility to further comprehend the mechanism of HCC occurrence,thereby to further consummate the research of tree shrew's HCC model.