Immunomodulatory of selenium nano-particles decorated by sulfated Ganoderma lucidum polysaccharides |
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| Institution: | 1. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China;2. School of Food and Pharmaceutical Engineering, Hubei University of Technology, Wuhan 430068, China;1. School of the Environment and Safety Engineering, Jiangsu University, 301 Xuefu, Rd., 212013 Zhenjiang, Jiangsu, China;2. School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China;3. School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China;4. School of Pharmacy, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China;5. Zhejiang Fangge Pharmaceutical Co., Ltd., Xingguang Rd., 323800 Qingyuan, Zhejiang, China;1. Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China;2. School for Radiological and Interdisciplinary Sciences (RAD-X), State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou, 215123, China;3. Department of General Surgery, Changshu No. 2 People’s Hospital, Changshu, 215500, China;4. Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, 215007, China;5. Zhejiang Reachall Pharmaceutical Co., Ltd., Dongyang, Zhejiang, 322100, China;6. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China;7. Shanghai R&D Centre for Standardization of Chinese Medicines, Shanghai, 201210, China |
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| Abstract: | In this study, we employed a one-step method to prepare selenium nanoparticles (SeNPs) decorated by the water-soluble derivative of Ganoderma lucidum polysaccharides (SPS). The SeNPs–SPS complexes were stable, and the diameter of the SeNPs was homogeneous at around 25 nm. We investigated the anti-inflammatory activity of SeNPs–SPS against murine Raw 264.7 macrophage cells induced by LPS. SeNPs–SPS were found to significantly inhibit LPS-stimulated nitric oxide (NO) production against Raw 264.7 macrophages. RT-PCR results reveal the down-regulation of mRNA gene expressions for pro-inflammatory cytokines, including inducible NO synthase (iNOS), interleukin (IL)-1 and TNF-α in a dose-dependent manner. However, the anti-inflammation cytokine IL-10 was markedly increased. In the NF-κB signal pathway, SeNPs–SPS significantly inhibited the phosphorylation of Iκ-Bα. Similar results were observed for inhibition of the phosphorylation of JNK1/2 and p38 mitogen-activated protein kinase(MAPKs), whereas ERK1/2 MAPK was not apparently affected by SeNPs–SPS. All of these results suggest that SeNPs–SPS complexes have anti-inflammatory potential modulating pro-/anti-inflammation cytokine secretion profiles, and that the mechanism is partially due to inhibition of activations of NF-κB, JNK1/2 and p38 MAPKs. |
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| Keywords: | Selenium nanoparticles Polysaccharides Anti-inflammation Signal pathways |
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