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| 大鼠主动脉粥样硬化斑块中Nogo-B的表达 | |
| 引用本文： | 潘静薇,魏盟,杨鹏远,李京波,陆志刚,郑兴,芮耀诚. 大鼠主动脉粥样硬化斑块中Nogo-B的表达[J]. 中华老年心脑血管病杂志, 2010, 12(2). DOI: 10.3969/j.issn.1009-0126.2010.02.018 |
| 作者姓名： | 潘静薇 魏盟 杨鹏远 李京波 陆志刚 郑兴 芮耀诚 |
| 作者单位： | 1. 上海交通大学附属第六人民医院心内科,200233 2. 第二军医大学附属药学院 3. 第二军医大学附属长海医院 |
| 摘 要： | 目的检测大鼠主动脉粥样硬化斑块中Nogo-B的表达。方法选取40只Wistar大鼠,随机分为对照组和实验组,每组20只。实验组制成动脉粥样硬化模型,对照组同期正常饲养,8周后处死大鼠,取主动脉部位,采用Northen Blot和免疫组织化学技术检测Nogo-B mRNA和蛋白水平的表达。结果与对照组比较,实验组大鼠主动脉粥样硬化斑块中内皮细胞Nogo-B蛋白表达降低(P0.05);Nogo-B1 mRNA表达显著下降40%,差异有统计学意义(P0.01),Nogo-B2 mRNA表达轻度下降,但差异无统计学意义(P0.05)。结论大鼠动脉粥样硬化斑块中Nogo-B1 mRNA和蛋白水平表达降低,推测Nogo-B1可能在动脉粥样硬化斑块形成过程中起保护作用。 |
| 关 键 词： | 动脉粥样硬化 内皮,血管 免疫组织化学 RNA,信使 |
Expression of Nogo-B in the atherosclerotic plaque of rat's aorta |
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| Abstract: | Objective To investigate the expression of Nogo-B in the atherosclerotic plaque of rat's aorta.Methods Forty Wistar rats were equally divided into 2 groups:control group and experiment group.The rats of experiment group were used to form atherosclerotic models.Protein and mRNA expression levels of Nogo-B were measured with Northern Blot and immunohistochemistry respectively.Results Tissue immunohistochemical staining of aortic specimens indicated that Nogo-B took part in neovascularization and proliferation of smooth muscle.Compared with control group,the expression of Nogo-B in endothelial cells of plaques of aorta of rats in experiment group decreased.The mRNA expression levels of Nogo-B1 in atherosclerotic plaques decreased by 40% compared with those in normal aorta.while the expression of Nogo-B2 changed slightly.Conclusion The expression of mRNA and protein of Nogo-B1 in atherosclerotic plaque is positively correlated to the formation of atherosclerosis and Nogo-B1 may have preventive effect against the pathological process of atherosclerosis. |
| Keywords: | atherosclerosis endothelium,vascular immunohistochemistry RNA,messenger |
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