| 第三代腺病毒载体的改良与应用 |
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| 引用本文： | 陈琳,薛绪潮.第三代腺病毒载体的改良与应用[J].第二军医大学学报,2007,28(7):0722-0725. |
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| 作者姓名： | 陈琳 薛绪潮 |
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| 作者单位： | 第二军医大学长海医院普通外科,上海,200433;第二军医大学长海医院普通外科,上海,200433 |
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| 摘 要： | 腺病毒载体是一种非常有效的转基因载体,具有高效率、低致病性、高滴度及不整合入宿主细胞染色体等优点,广泛应用于基因治疗.第一、二代腺病毒载体仍有许多不足之处,在其基础上发展了第三代腺病毒载体.第三代腺病毒载体与前者相比,具有低免疫原性及高容量等优点,在基因治疗中表现出独特优势.然而,由于辅助病毒污染的存在及病毒产量有待提高,第三代腺病毒载体的临床应用仍面临一定困难.
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| 关 键 词： | 第三代腺病毒 遗传载体 辅助病毒 |
| 文章编号： | 0258-879X（2007）07-0722-04 |
| 修稿时间： | 2007-01-232007-03-29 |
Modification and application of the third-generation adenovirus vectors |
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| CHEN Lin,XUE Xu-chao.Modification and application of the third-generation adenovirus vectors[J].Academic Journal of Second Military Medical University,2007,28(7):0722-0725. |
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| Authors: | CHEN Lin XUE Xu-chao |
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| Institution: | Department of General Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China |
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| Abstract: | Adenovirus as gene transfer vector is characterized by high transfection efficiency, high titer,good stability in target cells and low pathogenicity, and has been widely used in gene therapy. Compared to first- and second-generation adenovirus vectors, the third-generation adenovirus vectors have unique advantages for gene therapy because of their low immunogenicity and high capacity. However, the clinical application of the third-generation adenovirus vectors is hampered by the existance of helper contamination and difficulties in their large-scale production |
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| Keywords: | third-generation adenovirus genetic vectors helper viruses |
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