生脉注射液抗急性百草枯中毒大鼠自由基损伤作用的实验研究

目的 探讨生脉注射液对急性百草枯中毒的治疗作用.方法 将50只SD大鼠随机分为5组(空白组、阴性对照组、阳性对照组、生脉低剂量组和生脉高剂量组),每组各10只.空白组大鼠腹腔注射生理盐水30 ml/kg,其他组复制成百草枯急性中毒模型.观察大体标本、组织病理以及生物学标志(肺湿重/干重比、肺泡灌洗液中性粒细胞比和蛋白含量).同时测定血清和肺泡灌洗液中丙二醛含量以及超氧化物歧化酶和谷胱甘肽过氧化物酶活力.结果 与阴性对照组相比,生脉低剂量组肺组织病理显示肺淤血、肺水肿明显减轻,而且血浆和肺泡灌洗液中丙二醛含量分别由(5.04±0.50)、(1.19±0.18)nmol/ml下降至(4.04±0.21)、(0.79±0.04)nmol/ml,超氧化物歧化酶活力由(123.30±20.39)、(26.43±2.22)U/ml升高至(277.09±11.66)、(37.10±2.49)U/ml,谷胱甘肽过氧化物酶活力由(1796.63±81.12)、(598.24±62.50)U/ml升高至(2151.54±148.32)、(1788.44±175.11)U/ml;2组比较差异均有统计学意义(P均<0.01).结论 生脉注射液可使急性百草枯中毒引起的脂质过氧化损伤得到改善,低剂量效果更佳.

Experimental study on effect of pulse-actlvating injection on injury of free radical in acute paraquat poison-ing in rats

Objective To study the therapeutic effect of pulse-activating injection on acute poisoning by pa-raquat(PQ). Methods 50 Sprague-Dawley (SD) rats were randomly divided into five experimental groups: blank group, negative control group, positive control group, low-dose pulse-activating injection group (LDG), and high-dose pulse-activating injection group(HDG) (n = 10 for each group). Blank group were injected with normal suline,30 ml/kg,and other groups were established as acute paraquat poisoning models. Macroscopic and histopathological ex-aminations were performed and biological indexes were measured for the lung specimens. The indexes included lung wet weight/dry weight,the rats of neutrophils and protein content in the pulmonary alveolar lavage fluid. In the mean time, the level of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) both in plasma and bronchoalveolar lavage flui(BALF) were detected. Results Compared with that in negative control group,lung congestion and lung edema of LDG group were mitigated; and the MDA level decreased from (5.04± 0.50) nmol/ml,(1. 19±0.18) nmol/ml to (4.04±0.21) nmol/ml,(0.79±0.04) nmol/ml both in plasma and BALF;the SOD activities were increased from (123.30±20. 39) U/ml, (26.43±2.22) U/ml to (277.09± 11.66) U/ml,(37.10± 2.49) U/ml as well; the GSH-Px activities were increased from (1796.63 ±81. 12) U/ml, (598.24 ± 62.50) U/ml to (2151.54 ± 148.32) U/ml, ( 1788.44 ± 175.11 ) U/ml as well ( P < 0. 01 ). Conclusions Administration of pulse-activating injection could improve the lipid peroxidation damage caused by a-cute poisoning of PQ.