胰岛素受体底物-2的Gly1057Asp基因多态性与颈动脉粥样硬化性狭窄相关性的研究

目的 探讨胰岛素受体底物-2的Gly1057Asp基因多态性与颈动脉粥样硬化性狭窄的相关性。方法 选择96例行颈部血管彩超示颈动脉狭窄≥50%的北方汉族人为实验组以及同期79例体检健康者作为对照组,通过聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)技术检测所有入选患者的Gly1057Asp基因多态性,比较2组的等位基因频率、基因型频率以及Gly1057Asp基因多态性对颈动脉狭窄的影响。结果 实验组中G等位基因频率(78.6%)明显高于对照组(68.4%)(P<0.05); 实验组中G/A+A/A基因型频率(36.5%)明显低于对照组(51.9%)(P<0.05)。携带等位基因G的个体患有颈动脉粥样硬化性狭窄的风险是非等位基因G携带者的1.99倍(OR=1.994,95%CI=1.068～3.761)。结论 胰岛素受体底物-2的Gly1057Asp基因多态性可能与颈动脉粥样硬化性狭窄有关,等位基因G可能是颈动脉粥样硬化性狭窄的独立危险因素。

The association between Gly1057Asp polymorphism of insulin receptor substrate-2 and carotid

ObjectiveTo evaluate the association between the Gly1057Asp polymorphism of insulin receptor substrate-2 and carotid atherosclerosis. To explore the genetic mark of the susceptibility of carotid artery stenosis.Methods 96 Patients with greater than or equal to 50% carotid artery stenosis and 79 healthy controls who were the Han nationality in the north were choosed to detect the Gly1057Asp polymorphism by the polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)technique, and all of them had been received cervical vascular color ultrasonic inspection. The allele frequency, genotypes frequency between the two groups were compared. The relationship between Gly1057Asp polymorphism and carotid ar-tery stenosis was explored.Results The G allele frequency in the experimental group(78.6%)was significantly higher than that in the control group(68.4%)(P<0.05); The G/A+A/A genotypes in the experimental group(36.5%)were obviously lower than that in the control group(51.9%)(P<0.05). The patients with allele G had a 1.99-fold increased risk for carotid artery stenosiscompared with those people who had no allele G(OR=1.994, 95%CI 1.068-3.761).Conclusions The Gly1057Asp polymorphism of insulin receptor substrate-2 is probably associated with carotid artery stenosis,and the allele G may be arisk factor for carotid artery stenosis.