APETX2对氯化锂-匹鲁卡品诱导痫性发作大鼠的影响及机制探究

目的 探索APETX2对氯化锂-匹鲁卡品诱导痫性发作大鼠的行为学影响及可能的机制。方法 成年雄性SPF级SD大鼠18只,侧脑室置管后随机分为:癫痫组(9只)、APETx2组(9只),癫痫造模后观察2组癫痫大发作潜伏期及发作强度; APETx2处理原代培养海马神经元,动态观察其对钙成像的影响。结果 APETx2组的SD大鼠癫痫潜伏期延长,大发作程度减轻; APETx2处理原代培养海马神经元钙内流下降。结论 APETx2可抑制氯化锂-匹鲁卡品诱导SD大鼠痫性发作,减少酸诱导海马神经元钙离子浓度增加可能为机制之一。

Effects of APETX2 on ethology of rat seizures induced by lithium chloride - pilocarpine induced and its mechanism exploration

ObjectiveTo study the effects of APETX2 on ethology of lithium chloride-pilocarpine induced seizures rat and possible mechanism.Methods Adult male Specific pathogen Free Sprague Dawley rats(n=18)were randomly divided into two groups after lateral ventricle intubating:APETX2 group(n=9)and control group(n=9). The latencies and severity of epileptic seizure were compared between two groupsafter building epileptic model. The effects of APETX2 on calcium imaging about primary hippocampal neurons were dynamically observed.Results APETX2 obviously prolonged the latencies of epileptic seizure and reduced seizure degree. Primary hippocampal neurons' calcium imaginginflowing decreased significantly in APETX2 group.Conclusion APETX2 can inhibit lithium chloride-pilocarpine induced rat seizures. One of its mechanisms may be that APETX2 reduced calcium ionconcentration enhancement induced by acid in hippocampal neurons.