针吸型肌肉活检联合免疫荧光在假肥大型肌营养不良诊断中的应用

目的 探讨应用针吸型肌肉活检结合免疫荧光染色诊断假肥大型肌营养不良症的应用价值及意义。方法 应用针吸型活检术取533例假肥大型肌营养不良症患者(415例DMD, 118例BMD)的肌组织,采用HE染色观察肌细胞形态,免疫荧光染色技术检测抗肌营养不良蛋白, 以2 例正常人的肌细胞作为对照。结果 正常人肌细胞膜上抗肌萎缩蛋白染色阳性,可见沿肌细胞膜分布完整的荧光条带; DMD 患者肌膜染色阴性,肌细胞膜完全不显色; BM D患者染色弱阳性, 可见沿肌细胞膜分布的间断斑片状荧光带。结论 应用针吸型活检术联合免疫荧光染色可以有效的检测抗肌营养不良蛋白的表达, 有助于DMD 和BMD 的确诊及鉴别诊断。

The application of needle muscle biopsy combined with immunofluorescence in diagnosing of pseudohypertrophic muscular dystrophy

ObjectiveTo explore the application significance and value of needle muscle biopsy combined with immunoflurescence in diagnosing of pseudohypertrophic muscular dystrophy.Methods The muscle specimens were obtained from 533 patients(415 DMD and 118 BMD)with pseudohypertrophic muscular dystrophy by using needle biopsy. The sections were treated with HE stain, and the pathological changes were observed. Dystrophin in muscle cells was detected by immunofluorescence technique and compared with two normal muscle.Results The normal muscle showed ringed positive staining stripes around muscle cells. Negative result of staining was seen in DMD patients. The discontinuous positive staining was showed in BMD patients.Conclusion Detecting dystrophin in muscles cells by needle biopsy and immunofluorescence technique is an efficient method for diagnosising and distinguishing DMD and BMD.