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波形蛋白阳性表达在染锰大鼠睾丸中的定位研究
引用本文:才秀莲,王国秀,陈伟,郭海. 波形蛋白阳性表达在染锰大鼠睾丸中的定位研究[J]. 遵义医学院学报, 2009, 32(6): 542-544
作者姓名:才秀莲  王国秀  陈伟  郭海
作者单位:遵义医学院,组织学与胚胎学教研室,贵州,遵义,563000
摘    要:目的 研究波形蛋白(vimentin,VM)阳性表达在染锰大鼠睾丸中的定位。方法将正常雄性SD大鼠8只,给予大鼠腹腔注射15mg/kg氯化锰。染锰4wk,随机处死。免疫组织化学链酶亲和素-生物素-过氧化物酶复合物(streptavidin—biotin—peroxidasecomplex,SABC)法进行检测研究。结果在染锰大鼠睾丸中除间质,间质细胞、支持细胞,肌样细胞细胞质波形蛋白表达阳性外,也有精原细胞的细胞质VM表达阳性。结论染锰大鼠睾丸的某些精原细胞的细胞质VM表达阳性。

关 键 词:  大鼠  睾丸  精原细胞  波形蛋白

Study of localization of vimentin positive expression in Rats testis exposed to Manganese
CAI Xiu-lian,WANG Guo-xiu,CHEN Wei,GUO Hai. Study of localization of vimentin positive expression in Rats testis exposed to Manganese[J]. Acta Academiae Medicine Zunyi, 2009, 32(6): 542-544
Authors:CAI Xiu-lian  WANG Guo-xiu  CHEN Wei  GUO Hai
Affiliation:(Department of Histology and Embryology,Zunyi Medical College,Guizhou Zunyi 563000,China)
Abstract:Objective To study localization of vimentin positive expression in Rats testis exposed to Manganese. Methods The 8 male rats exposed to 15 mg/kg manganese for 4 week, the expression of vimentin in rats testis was detected by immunohistoehemitry (SABC). Results The expression of vimentin in interstitial tissue, cytoplasm of interstitial cells, cytoplasm of Sertoli, eytoplasm of myoid eells were positive, not excepting cytoplasm of spermatogoniums. Conclusions The expression of vimentin in cytoplasm of spermatogoniums was positive.
Keywords:manganese  rat  testis  spermatogoniums  vimentin
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