基质金属蛋白酶-2、7在结直肠癌中的表达及临床意义

目的 探讨结直肠癌中基质金属蛋白酶-2、7(MMP-2、MMP-7)的表达及其临床意义.方法 采用荧光定量逆转录PCR(FQ-RT-PCR)法和免疫组化法检测42例结直肠癌组织及其邻近正常黏膜组织中MMP-2 mRNA和MMP-7 mRNA及其蛋白,并分析其与结直肠癌临床病理特征的关系.结果 结直肠癌组织MMP-2 mRNA、MMP-7 mRNA的转录水平和MMP-2、MMP-7的表达水平均显著高于邻近正常黏膜组织(P均<0.05),且均与浸润深度、淋巴结转移、Dukes分期正相关.结直肠癌组织MMP-2 mRNA转录水平和MMP-7 mRNA转录水平、MMP-2表达水平和MMP-7表达水平均呈正相关(rs=0.454,P=0.003;rs=0.343,P=0.026).结论 MMP-2和MMP-7在结直肠癌发生发展中起重要作用,联合检测二者有助于结直肠癌的恶性程度及生物学行为的判断及预后评估.

The expression and clinical significance of MMP-2 and MMP-7 in colorectal carcinomas

Objective To investigate the expression of MMP-2 and MMP-7 and their elinieopathologieal significance in eoloreetal carcinomas. Methods The expression of MMP-2 mRNA, MMP-7 mRNA and MMP-2, MMP-7 in 42 samples of eoloreetal carcinomas and its adjacent normal eoloreetal mueosa were examined using fluo-rescence quantitative RT-PCR (FQ-RT-PCR) and immunohistoehemistry. The relationship of their expression with some elinieopathologieal characteristics was analyzed. Results MMP-2 mRNA, MMP-7 mRNA and MMP-2, MMP-7 were significantly over-expressed in eoloreetal carcinomas compared with its adjacent normal eoloreetal mueosa( P < 0.05 ) , and they were positively associated with bowel wall invasion, the regional lymph node metastasis and Dukes stage. The expression of MMP-2 mRNA and MMP-7 mRNA was positively correlated with MMP-2 and MMP-7 in eoloreetal eareinornas. Conclusions MMP-2 and MMP-7 may play a key role in eoloreetal carcinogenesis,tumor in-vasion and metastasis. Examination of combined MMP-2 and MMP-7 expression may have an important significance to judge the malignant degree and biological behavior of human eoloreetal carcinoma and for evaluating the progno-sis.