Nanoparticle Coated Submicron Emulsions: Sustained In-vitro Release and Improved Dermal Delivery of All-trans-retinol |
| |
Authors: | Ghouchi Eskandar Nasrin Simovic Spomenka Prestidge Clive A |
| |
Institution: | (1) Ian Wark Research Institute, ARC Special Research Centre for Particle and Material Interfaces, University of South Australia, Adelaide, South Australia, 5095, Australia |
| |
Abstract: | Purpose The aim of this research is to investigate the dermal delivery of all-trans-retinol from nanoparticle-coated submicron oil-in-water emulsions as a function of the initial emulsifier type, the loading
phase of nanoparticles, and the interfacial structure of nanoparticle layers.
Methods The interfacial structure of emulsions was characterized using freeze-fracture-SEM. In-vitro release and skin penetration of all-trans-retinol were studied using Franz diffusion cells with cellulose acetate membrane, and excised porcine skin. The distribution
profile was obtained by horizontal sectioning of the skin using microtome-cryostat and HPLC assay.
Results The steady-state flux of all-trans-retinol from silica-coated lecithin emulsions was decreased (up to 90%) and was highly dependent on the initial loading phase
of nanoparticles; incorporation from the aqueous phase provided more pronounced sustained release. For oleylamine emulsions,
sustained release effect was not affected by initial location of nanoparticles. The skin retention significantly (p ≤ 0.05) increased and was higher for positive oleylamine-stabilised droplets. All-trans-retinol was mainly localized in the epidermis with deeper distribution to viable skin layers in the presence of nanoparticles,
yet negligible permeation (∼1% of topically applied dose) through full-thickness skin.
Conclusions Sustained release and targeted dermal delivery of all-trans-retinol from oil-in-water emulsions by inclusion of silica nanoparticles is demonstrated. |
| |
Keywords: | all-trans-retinol in-vitro release silica nanoparticles skin penetration/permeation submicron emulsion |
本文献已被 SpringerLink 等数据库收录! |
|