利用荧光探针JC-1检测心肌细胞线粒体膜电位的改变

利用荧光探针JC－1观察大鼠心肌细胞凋亡早期线粒体膜电位的变化。用过氧化氢（H2O2）诱导心肌细胞凋亡，在凋亡早期，利用新型荧光探针JC－1标记细胞线粒体，在FACS Calibur流式细胞仪上检测JC－1荧光强度的变化，用Annexin-V/PI双染色法鉴别凋亡和坏死的心肌细胞。结果显示，在正常大鼠心肌细胞中，线粒体维持较高的膜电位，JC－1在线粒体内形成聚合物，发出红色荧光。H2O2导致线粒体膜电位下降，JC－1聚合物分解成单体，红色荧光强度减弱。提示JC－1结合流式细胞仪是一种理想的检测线粒体膜电位的手段。

DETECTION OF MITOCHONDRIAL MEMBRANE POTENTIAL CHANGES IN CARDIOMYOCYTES BY FLUORESCENT DYES JC-1

To explore the changes of mitochondrial membrane potential in rat cadiomyocyte apoptosis by fluorescent JC 1, H 2 O 2 was used to induce cadiomyocyte apoptosis, and JC 1 in combination with flow cyometry was used to detect the changes of mitochondrial membrane potential in the early stage of cadiomyocyte apoptosis. The results showed that living cardiomyocytes had a high mitochondrial membrane potential, JC 1 aggregates were formed in the inner membrane of mitochondria and emitted orange red fluorescence. H 2 O 2 caused the decrease of mitochondrial membrane potential, JC 1 aggregates were dissociated to monomer,which emitted green fluorenscence. So the red fluorescence decreased. It is suggested that JC 1 in combination with flow cyometry is an ideal method to detect the changes of mitochondrial membrane potential.