Antioxidant activity of yellow dock (Rumex crispus L., Polygonaceae) fruit extract |
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| Authors: | Zoran Maksimović Nada Kovačević Branislava Lakušić Tatjana Ćebović |
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| Affiliation: | 1. Institute of Pharmacognosy, School of Pharmacy, University of Belgrade, Belgrade, Serbia;2. Institute of Botany, School of Pharmacy, University of Belgrade, Belgrade, Serbia;3. Institute of Biochemistry, School of Medicine, University of Novi Sad, Novi Sad, Serbia |
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| Abstract: | The methanol extract of ripe Rumex crispus L. fruits was evaluated for its antioxidant potential by assays for ferric‐reducing antioxidant power (FRAP), DPPH‐free radical scavenging activity (DPPH) and the influence on lipid peroxidation in liposomes (LP). Considerable activity was observed in all test systems (FRAP: 9.9 mmol Fe2+/g; DPPH IC50: 3.7 μg/mL; LP IC50: 4.9 μg/mL), comparable to that of BHT (FRAP: 8.0 μg/mL; DPPH IC50: 19.4 μg/mL; LP IC50: 3.5 μg/mL), but lower than the activity of ascorbic acid, rutin and quercetin, used as positive control substances. The in vivo effects were evaluated in several hepatic antioxidant systems (activities of LPx, GSH‐Px, Px, CAT and XOD, as well as GSH content), after treatment with the studied yellow dock extract in different doses, or in combination with carbon tetrachloride (CCl4). Pretreatment with the R. crispus extract inhibited CCl4‐induced oxidative stress by decreasing LPx and increasing GSH content in a dose dependent manner, bringing the levels of antioxidant enzymes to near control values. Copyright © 2010 John Wiley & Sons, Ltd. |
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| Keywords: | Rumex crispus L. antioxidant activity ferric‐reducing antioxidant power DPPH free radical scavenging activity lipid peroxidation |
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