11Beta-Hydroxysteroid Dehydrogenase Messenger Ribonucleic Acid Expression, Bioactivity and Immunoreactivity in Rat Cerebellum |
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| Authors: | Marie-Pierre Moisan Jonathan R. Seckl Lawrence P. Brett Carl Monder Anil K. Agarwal Perrin C. White Christopher R. W. Edwards |
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| Affiliation: | Department of Medicine, Western General Hospital, Edinburgh EH4 2XU, UK.;Departments of Medicine and Pathology, Western General Hospital, Edinburgh EH4 2XU, UK.;The Population Council, Center for Biomedical Research, 1230 York Avenue, New York, New York 10021, USA.;Division of Paediatrics, New York Hospital, New York Medical School, New York 10021, USA. |
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| Abstract: | 11β-Hydroxysteroid dehydrogenase (11β-OHSD) metabolizes corticosterone to inactive 11-dehydrocorticosterone and thus protects non-specific mineralocorticoid receptors from exposure to corticosterone in the kidney in vivo. Clearly, 11β-OHSD might also regulate corticosterone access to glucocorticoid receptors. We have investigated cerebellum, a tissue with high glucocorticoid receptor, but very low mineralocorticoid receptor levels and have shown marked 11β-OHSD bioactivity with similar co-substrate requirements and inhibition kinetics to the renal enzyme. 11β-OHSD messenger ribonucleic acid was expressed in cerebellum and was localized in Purkinje and granule cells. This distribution was confirmed immunohistochemically. Thus, we provide evidence for 11β-OHSD in cerebellum and suggest that it may regulate the access of corticosterone to glucocorticoid receptors in addition to mineralocorticoid receptors. |
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| Keywords: | 11β-hydroxysteroid dehydrogenase cerebellum steroid receptors corticosterone in situ hybridization |
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