| 人包皮脱细胞基质组织工程化尿道的可行性研究 |
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| 引用本文： | 姜华,马利民,周娟,卢慕峻,蔡志康,王浩,达骏,陈其,王忠.人包皮脱细胞基质组织工程化尿道的可行性研究[J].中华男科学杂志,2009,15(5):409-412. |
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| 作者姓名： | 姜华 马利民 周娟 卢慕峻 蔡志康 王浩 达骏 陈其 王忠 |
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| 作者单位： | 上海交通大学医学院附属第九人民医院泌尿外科,上海,200011 |
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| 基金项目： | 国家自然科学基金，上海浦江人才计划，上海市科委基础研究重点项目，上海市科委环境条件计划，上海市教委科研创新项目，上海高校选拔培养优秀青年教师科研专项基金，上海交通大学医学院科技基金 |
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| 摘 要： | 目的:为人包皮脱细胞基质作为尿道组织工程修复材料提供依据。方法:制备人包皮脱细胞基质,对其进行组织学观察、细胞毒性以及体内实验,检验其作为尿道材料的安全性和组织相容性。结果:制备的人包皮脱细胞基质,细胞去除完全,用原代包皮上皮细胞测定其细胞毒性,结果显示细胞相对增值率在75%～99%之间,细胞毒性为1级,符合国家标准,回植体内后,随着时间的延长,人包皮脱细胞基质与尿路上皮细胞结合完好,正常尿道复层结构层次逐步恢复。结论:人包皮脱细胞基质抗原性低,相容性好,可以作为组织工程尿道的支架材料。
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| 关 键 词： | 包皮 脱细胞基质 尿道 组织工程 |
Human Foreskin Acellular Matrix Graft: A Good Scaffold for Urethral Tissue Engineering |
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| JIANG Hua,MA Li-min,ZHOU Juan,LU Mu-jun,CAI Zhi-kang,WANG Hao,DA Jun,CHEN Qi,WANG Zhong.Human Foreskin Acellular Matrix Graft: A Good Scaffold for Urethral Tissue Engineering[J].National Journal of Andrology,2009,15(5):409-412. |
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| Authors: | JIANG Hua MA Li-min ZHOU Juan LU Mu-jun CAI Zhi-kang WANG Hao DA Jun CHEN Qi WANG Zhong |
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| Institution: | JIANG Hua,MA Li-min,ZHOU Juan,LU Mu-jun,CAI Zhi-kang,WANG Hao,DA Jun,CHEN Qi,WANG ZhongDepartment of Urology,The Ninth People's Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200011,China |
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| Abstract: | Objective: To provide evidence of using the human foreskin acellular matrix graft for urethral tissue engineering. Methods: The human foreskin acellular matrix graft was prepared,its safety and biocompatibility as urethral material were determined by histological observation,cytotoxicity test using primary epithelial cells and experiment in vivo.Results: Intact cells were absent from the foreskin acellular matrix graft.The cytotoxicity test showed that the relative growth rate of the cells was between 75% a... |
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| Keywords: | foreskin acellular matrix graft urethra tissue engineering |
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