结核分枝杆菌环介导恒温扩增（LAMP）快速检测方法的评价

目的 对国内建立的结核分枝杆菌DNA环介导恒温扩增（loop-mediated isothermal amplification, LAMP）快速检测方法进行了介绍和评价。 方法 实验菌株来源于北京结核病胸部肿瘤研究所。以结核分枝杆菌作为研究对象，设计了4 条LAMP 引物特异地识别结核分枝杆菌中 gyr B 基因的6个特殊区域。所有的菌株样本提取DNA作为模板，加入LAMP反应管后，65 ℃恒温反应60 min即可。其结果可以通过肉眼观察到的颜色变化来判断。 结果 实验共验证了79株临床和标准菌株。对于菌株的培养物，LAMP实验的灵敏度为100%，特异度为92%。 结论 结核分枝杆菌LAMP快速检测方法是一种快速、可靠的结核分枝杆菌诊断方法。

Evaluation of loop-mediated isothermal amplification(LAMP)in rapid detection of Mycobacterium tuberculosis

Objective To evaluate the value of loop-mediated isothermal amplification（LAMP） in rapid detection of Mycobacterium tuberculosis.Methods A set of four LAMP primers were designed for recognizing six distinct regions on the target DNA（gyrB）.The DNAs of M.tuberculosis strains from Beijing Chest Hospital were extracted,amplified at 65°C for 60 min,and their reaction results were observed by simple naked eye.Results A total of 79 clinical isolates and standard strains were studied.The sensitivity and specificity of LAMP was 100% and 92%,respectively.Conclusions LAMP is a rapid and reliable alternative method for diagnosing M.tuberculosis infection.