莱菔硫烷对A549细胞株CYR61基因表达的影响

 目的 探讨莱菔硫烷(sulforaphane, SFN)对A549细胞株CYR61基因表达的影响。 方法 用MTT法检测SFN对A549细胞的抑制效应。用real-time PCR检测SFN干预的A549肺癌细胞株CYR61表达水平。 结果 MTT结果显示：SFN从40 μM的浓度起开始出现细胞抑制效应，随着浓度的升高呈现剂量依赖效应，浓度越高抑制率越大；且40 μM、80 μM、100 μM和160 μM SFN等较大剂量在不同作用时间点具有时间效应关系，干预时间越长，抑制率越大。光镜下细胞形态： 细胞在培养32 h后， 25 μM的SFN处理组部分A549细胞皱缩、变圆，折光性上升；50 μM的SFN处理组皱缩细胞数目增多；75μM的SFN处理组细胞皱缩数目进一步增多，部分细胞漂浮，并可见细胞碎片。25 μM和50 μM浓度SFN干预组的CYR61基因表达量较高，25 μM SFN组与50 μM SFN组之间差异没有统计学意义，25 μM SFN组和50 μM SFN组与其他各组相比，差异有统计学意义。 结论 SFN在一定浓度下对A549细胞株起抑制作用，并对A549肺癌细胞株CYR61基因的表达有促进作用，可为食用十字花科类蔬菜预防肺癌提供依据。

Effects of SFN on Expression of CYR61 in A549 Cell Lines

Objective To study the effects of SFN on A549 cell lines through analyzing inhibitory rate and the CYR61 expression of A549 cell. Methods The inhibition of SFN on A549 was detected by MTT and CYR61 expression level intervened by SFN was identified by real-time PCR. Results MTT results indicated that SFN had a restrain effect in a dose-dependent manner when the concentration exceeded 40 μM. For the inhibitory ratio, it was in a time dependent among the dose groups of 40 μM, 80 μM, 100 μM, and 160 μM. A549 cells treated with SFN for 32 hour shown crimped under the microscope and high refraction quantity cell configuration at 25 μM; the amount of crimpled cells was increased at 50 μM; part of the cells were floating and became to fragments at 75 μM. The expression of CYR61 was higher in the group of 25 μM and 50 μM. There was no significant difference between 25 μM and 50 μM group. The expression of CYR61 was lower in 75 μM group than that in 50 μM group. Conclusion SFN inhibited growth of A549 cells in a does-dependent manner and promoted the expression of CYR61. So distilled SFN may be a preventive factor for lung cancer development.