| 不同年龄人骨髓间充质干细胞体外增殖及成骨分化的研究 |
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| 引用本文： | 朱灏,许建中,周强,王序全,单建林,赵敏,秦辉.不同年龄人骨髓间充质干细胞体外增殖及成骨分化的研究[J].中国矫形外科杂志,2004,12(10):759-761. |
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| 作者姓名： | 朱灏 许建中 周强 王序全 单建林 赵敏 秦辉 |
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| 作者单位： | 第三军医大学附属西南医院骨科,400038 |
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| 基金项目： | 国家 8 63计划 (2 0 0 3AA2 0 50 0 2 )，重庆市科技攻关基金资助重点项目 (2 0 0 3 - 1 3 - 6)，第三军医大学科研基金资助项目 (XG2 0 0 32 7) |
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| 摘 要： | 目的 :观测年龄对人骨髓间充质干细胞 (humanmesenchymalstemcells ,hMSCs)体外增殖、成骨分化的影响。方法 :使用密度梯度离心法分离不同年龄人骨髓MSCs进行培养 ,保留贴壁细胞传代 ,观察细胞生长情况 ,检测其增殖活性、碱性磷酸酶活性 (ALP)、诱导后骨钙素定量测定。结果 :低龄hMSCs较高龄hMSCs体外生长快、MTT、ALP及骨钙素浓度高。结论 :hMSCs的增殖和成骨分化的能力和活性随着年龄的增加而降低。
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| 关 键 词： | 骨髓间充质干细胞 年龄 细胞培养 增殖 成骨分化 |
| 文章编号： | 1005-8478(2004)10-0759-03 |
| 修稿时间： | 2003年12月3日 |
Effects of age on proliferation and osteogenic differentiation of human mesenchymal stem cells in vitro |
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| ZHU Hao,XU Jian-zhong,ZHOU Qiang,et al..Effects of age on proliferation and osteogenic differentiation of human mesenchymal stem cells in vitro[J].The Orthopedic Journal of China,2004,12(10):759-761. |
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| Authors: | ZHU Hao XU Jian-zhong ZHOU Qiang |
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| Institution: | ZHU Hao,XU Jian-zhong,ZHOU Qiang,et al.Department of Orthopedics,Southwest Hospital,the Third Military Medical University,Chongqing 400038 |
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| Abstract: | Objective: To determine whether or not the donor age influences proliferation and differentiation capability of human mesenchymal stem cells(hMSCs).Method:HMSCs cultures were obtained by using density centrifuge method from 39 donors between 7 and 79 years of age.The adhensive cells were preserved to passage culture,then the proliferation and osteogenic differentiation capability were investigated.Result:The total culture time was positively increased with donor age.Cell cultures from younger donors showed a higher activity of MTT and expression of alkaline phosphatase(ALP) and osteocalcin than that from older donors.Conclusion:The proliferation and osteogenic differentiation capability of hMSCs decreases with age.These findings have significant implications for the proposed clinical application of hMSCs. |
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| Keywords: | Mesenchymal stem cells Aging Cell culture Proliferation Osteogenic differentiation |
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