小半夏加茯苓汤中药效物质的正交试验筛选

目的确定小半夏加茯苓汤中药效物质。方法采用L9(34)正交试验制成不同提取工艺的处方,测定呕吐潜伏期、呕吐次数和总生物碱的质量分数,对数据进行线性回归。结果呕吐潜伏期与总生物碱呈良好线性关系,呕吐次数与总生物碱也呈良好线性关系。结论小半夏加茯苓汤中总生物碱是其药效物质。

Selecting for effective substance in Xiaobanxia Plus Fuling Decoction by orthogonal test

Objective To study the reaction mechanism of ginsenosides in red ginseng processing. Methods The major constituents of ginsenosides were assigned by HPLC/MS/MS data. Ginsenosides (including protopanaxadiol, protopanaxatriol, and oleanolic acid type) of white ginseng and red ginseng were compared by HPLC/MS data. Results Ginsenosides were produced from esterolysis of corresponding malonyl ginsenosides in red ginseng processing. Another reaction of malonyl ginsenosides in red ginseng processing was decarboxylated to produce corresponding acetyl ginsenosides. Hydrolysis of glycosidic bond at C_(20) and isomerous reaction of C_(20) were the main reaction of dammarane type ginsenoside. High degree of esterolysis and glycosidic bond hydrolysis of oleanolic acid type ginsenosides all occurred during the red ginseng processing. Conclusion Great changes of ginsenosides have been obviously found in processing ginseng to red ginseng. Special constituents of red ginseng could be produced in ginseng processing.