猪腹部多脏器移植术中内分泌功能的变化

目的；以美国油卡猪为研究对象，测定腹部多脏器联合移植术（ＭＯＴ）中ＡＣＴＨ、皮质醇、胰岛素、Ｃ肽血浆浓度的动态变化，为临床开展肝肠胰联合移植提供参数。方法；移植器官包括肝、部分有、十二指肠、胰腺及部分小肠。分别在术前、切除内脏后５分钟和３０分钟、再灌注前１０分钟、再灌注后１０分钟、术毕等六个时间点抽取颈内静脉血，采用放射免疫法测定ＡＣＴＨ、皮质醇、胰岛素、Ｃ肽的血浆浓度。结果：ＡＣＴＨ和皮质醇浓度

Changes of Plasmic Hormone during Orthotopic Abdominal Multiple Organ Transplantation

Objective:To determine the plasma ACTH,cortisol,insulin and C peptide during abdominal multiple organ transplantation.Methods:Transplantation organs included the liver,gallbladder,pancreas,duodenum,partial stomach and segments of the intestine.Blood specimens were drawn from the jugular vein before operation,at 5 and 30 min after abdominal viscera harvest(AVH),10min before reperfusion of blood,10min after reperfuson and the end of the operation.Plasma ACTH,cortisol,insulin and C peptide were detected by radioimmunoassay (RIA).Results:ACTH and cortisol were raised gradually at the beginning of the operation and kept at high levels thereafter,which was significantly different from those of preoperation ( P< 0 05).The levels of insulin and C peptide began to decrease after removal of abdominal organs and disappeared completely 30min later,and then raised gradually as the grafts were reperfused,but remained below the preoperative levels.Conculsions:The stress responses are very strong during MOT operation.Blood insulin may be completely exhausted within 30min after removal of the pancreas to the reperfusion of transplanted pancreas.