基于网络药理学的“淫羊藿-熟地黄”配伍治疗骨质疏松症作用机制研究

目的基于网络药理学方法探究"淫羊藿-熟地黄"药对治疗骨质疏松症(osteoporosis,OP)的分子机制。方法BATMAN-TCM数据库收集"淫羊藿-熟地"药对化学成分及对应靶点; Gene Cards、OMIM数据库收集OP靶蛋白; Venny 2.1. 0在线工具找出映射,绘制韦恩图; STRING 11.0数据库建立蛋白质相互作用网络(protein-protein interaction,PPI); cluster Profiler R包对所得共有靶点进行GO功能富集和KEGG通路富集分析; Cytoscape3.7. 2软件构建相关数据分析网络。结果获得"淫羊藿-熟地黄"药对中13个主要化学成分和134个蛋白靶点,OP相关基因3 231个,映射后获得药物与疾病共有靶点57个,PPI分析显示前列腺素内过氧化物合酶(PTGS2)、雄激素受体(AR)、雌激素受体(ESR1)等可能是"淫羊藿-熟地黄"药对治疗OP的核心靶点。GO富集分析包含血液循环、细胞钙离子稳态、G蛋白偶联胺受体活性等生物功能。KEGG通路富集分析主要包括神经活性配体-受体相互作用、类固醇激素的合成、c AMP信号通路等11条相关信号通路。结论 "淫羊藿-熟地黄"配伍通过多途径、多靶点发挥治疗OP的作用。

Study on the mechanism of the combination of herba epimedium and radix rehmanniae praeparata in the treatment of osteoporosis based on network pharmacology

Objective To explore the molecular mechanism and key targets of herba epimedium-radix rehmanniae praeparata in the treatment of osteoporosis (OP) based on network pharmacology. Methods The chemical constituents and corresponding targets of herba epimedium-radix rehmanniae praeparata were collected by using BATMAN-TCM database. Gen Cards OMIM database was used to collect OP target proteins. Venny 2.1.0 online tools was used to find relevant maps and draw Wayne diagram. STRING 11.0 database was used for the establishment of protein interaction network. The clusterProfiler R package was used for GO function enrichment and KEGG pathway enrichment analysis of the data. Cytoscape 3.7.2 software was used to build relevant data analysis network. Results Thirteen main chemical compositions, 134 protein targets, and 3231 OP-related genes in herba epimedium-radix rehmanniae praeparata were obtained. There were 57 targets after mapping between drug targets and disease targets. PPI protein interaction network analysis found PTGS2, AR, and ESR1 might be the core targets of epimedium-radix rehmanniae praeparata in the treatment of OP. GO enrichment analysis included blood circulation, homeostasis of calcium iron in the cell, and the activity of G-protein receptor. Enrichment analysis of the functional KEGG pathway identified 11 related signaling pathways including the neural ligand-receptor interaction, the cAMP signaling pathway, and the synthesis of steroid hormones. Conclusion The combination of herba epimedium and radix rehmanniae praeparata can play a role in the treatment of OP through multiple channels and multiple targets.