Crystallization of Cyclophosphamide Monohydrate During Lyophilization |
| |
Authors: | Bhushan Munjal Sandeep S. Zode Arvind K. Bansal |
| |
Affiliation: | Department of Pharmaceutics, NIPER SAS Nagar, sector-67, Mohali 160062, Punjab, India |
| |
Abstract: | The purpose of this study was to investigate the phase behavior of cyclophosphamide (CPA) during various stages of lyophilization, with special emphasis on obtaining crystalline CPA monohydrate (CPA-MH) in the lyophilized product. Subambient differential scanning calorimetry and low-temperature X-ray diffractometry (LTXRD) were used to study the phase behavior of CPA solution (3.7% w/v). In situ lyophilization in LTXRD chamber was used to monitor the phase transitions occurring during the drying stages. Finally, the implications of these findings were confirmed by freeze-drying the aqueous solution in a laboratory-scale freeze-dryer. The results suggested that CPA remains amorphous during freeze concentration, with a Tg' of ?50°C. However, its crystallization as CPA-MH can be induced by annealing the frozen solution between ?5°C and ?10°C. In situ lyophilization in LTXRD showed that the CPA-MH crystallized during annealing, rapidly dehydrated during primary drying, thereby causing structural collapse. The dehydration of CPA-MH can be prevented by lowering the escaping tendency of water molecules from the crystal lattice of CPA-MH by maintaining the chamber pressure to 300, 400, or 500 mTorr. This study highlights the relationship of process parameters used during lyophilization with the solid form of lyophilized CPA. |
| |
Keywords: | lyophilization freeze-drying processing crystallization hydrates dehydration CPA cyclophosphamide DSC differential scanning calorimetry LTXRD low-temperature X-ray diffractometry MH monohydrate XRPD X-ray powder diffraction |
本文献已被 ScienceDirect 等数据库收录! |
|