大鼠迟发性脑血管痉挛基底动脉不同时相的形态学改变

目的探讨大鼠枕大池二次注血蛛网膜下腔出血后20天内发生脑血管痉挛的基底动脉血管形态的动态变化规律。方法84只SD大鼠随机分为蛛网膜下腔出血组和枕大池注射NS对照组,采用枕大池二次注血的方法建立SAH型,NS组同法注射等量的NS;在首次注血或注水后0天(正常对照)、4、7、10、13、16天及20天,每组各灌注处死6只大鼠,取基底动脉行HE染色,测量其内径周长和血管壁厚度。结果脑血管痉挛在第4天已经出现,第7天达到高峰,高峰期持续至第10天,然后逐渐缓解,至第20天时,已接近正常对照组水平。结论大鼠迟发性脑血管痉挛形态学的时相变化为迟发性脑血管痉挛的研究提供了完整可靠的资料和方法。

Time course of basilar artery morphological changes of delayed cerebral vasospasm in rat

Objective To explore dynamic histological changes of hematoxylin-eosin-stained vasospastic basilar arteries from a "two-hemorrhage" rats model until 20 days after SAH.Methods 84 Sprague-Dawley rats were divided into group SAH(n=42) and saline control group(NS,n=42).SAH were developed with two times injections of 0.3 ml arterial blood into cisterna magna,and 0.3 ml saline was injected in group NS by using same method.The basilar arteries of 6 rats were taken for measurements of artery perimeter and wall thickness at 0(normal control),4,7,10,13,16,and 20 days after blood or water injection for each group respectively.Results It was observed that delayed cerebral vasospasm appeared at the fourth day after the first cisterna magna injection and reached the peak in the seventh day.The peak of vasospasm continued until the tenth day and then gradually diminished;perimeter returned to the normal control group on twentieth day.Conclusion The time course of cerebral vasospasm in histological changes can provide more reliable and more complete information about the alteration of cerebral artery in rats for the delayed cerebral vasospasm research.