| 阿尔茨海默病转基因鼠模型的建立 |
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| 引用本文： | 廖峥嵘,雷宇华,赵娟,吕雨虹,王忠海,张凤云,赵俊霞.阿尔茨海默病转基因鼠模型的建立[J].中国老年学杂志,2011,31(7). |
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| 作者姓名： | 廖峥嵘 雷宇华 赵娟 吕雨虹 王忠海 张凤云 赵俊霞 |
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| 作者单位： | 河北医科大学基础医学院细胞生物学教研室,河北,石家庄,050017 |
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| 基金项目： | 河北省科技厅科技支撑计划 |
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| 摘 要： | 目的 建立阿尔茨海默病(AD)双转基因鼠,为进一步研究AD发病机制提供较为理想的实验动物.方法 将人载脂蛋白E4转基因鼠和突变APP转基因鼠杂交.结果 经PCR初筛,对阳性小鼠基因组DNA作进一步的Southern杂交鉴定,获得2只双转基因小鼠,之后传代建系.结论 为进一步研究多个基因对AD的致病作用提供较为理想的实验动物.
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| 关 键 词： | APP基因 ApoE4基因 转基因动物 阿尔茨海默病 |
Study on Alzheimer's disease in transgenic mice |
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| LIAO Zheng-Rong,LEI Yu-Hua,ZHAO Juan,et al..Study on Alzheimer's disease in transgenic mice[J].Chinese Journal of Gerontology,2011,31(7). |
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| Authors: | LIAO Zheng-Rong LEI Yu-Hua ZHAO Juan |
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| Institution: | LIAO Zheng-Rong,LEI Yu-Hua,ZHAO Juan,et al.Department of Cell Biology,School of Basic Medical Sciences,Hebei Medical University,Shijiazhuang 050017,Hebei,China |
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| Abstract: | Objective To establish animal model of Alzheimer's disease(AD) in transgenic mice and investigate the mechanism of AD.Methods To establish AD bi-transgenic mice by ApoE4 and APP transgenic mice intercrossing.The PCR and Southern blot hybridization techniques were used for identification of transgenic mice.Results Two ApoE4/APP transgenic mice were born and young mice were bred.Conclusions ApoE4 and APP transgenic mice is a good AD animal model. |
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