| 大鼠脊髓冲击伤模型制作方法的改良探索 |
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| 引用本文： | 张小宁,张起顺,李忠伟,王宝兰.大鼠脊髓冲击伤模型制作方法的改良探索[J].中国临床神经科学,2011,19(3):302-306. |
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| 作者姓名： | 张小宁 张起顺 李忠伟 王宝兰 |
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| 作者单位： | 1. 新疆医科大学第一附属医院,神经内科,830054
2. 新疆医科大学第一附属医院,康复科,830054 |
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| 摘 要： | 目的:探索一种制作稳定性强、重复性好的脊髓损伤模型的简易方法。方法:应用简易重物坠落打击装置建立大鼠脊髓中度损伤组(5g×8cm)、重度损伤组(5g×16cm)模型和假手术组,每组大鼠均n=10。通过运动功能评分法(BBB)评分、苏木精-伊红染色观察脊髓损伤后两组大鼠功能及病理的变化特点,以评价该制作方法的可靠性。结果:BBB评分显示两组大鼠后肢运动功能均有不同程度的恢复,但中度损伤大鼠的恢复明显优于重度损伤大鼠(P<0.05)。组织病理学观察显示脊髓损伤后脊髓结构紊乱,有胶质瘢痕及空洞形成,重度损伤组脊髓空洞面积明显大于中度组(P<0.05)。结论:该方法制作的大鼠脊髓模型能将不同打击力度造成的损伤区分开,并且模型的行为学与病理学结果相吻合,说明此模型具有良好的稳定性、重复性和一致性,适合脊髓损伤实验研究的应用。
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| 关 键 词： | 大鼠 脊髓损伤 模型 |
Improved Probe on the Manufacturing Method of Spinal Cord Injury Model of Rat |
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| ZHANG Xiao-Ning,ZHANG Qi-Shun,LI Zhong-Wei,WANG Bao-Lan.Improved Probe on the Manufacturing Method of Spinal Cord Injury Model of Rat[J].Chinese Journal of Clinical Neurosciences,2011,19(3):302-306. |
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| Authors: | ZHANG Xiao-Ning ZHANG Qi-Shun LI Zhong-Wei WANG Bao-Lan |
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| Institution: | ZHANG Xiao-Ning1,ZHANG Qi-Shun1,LI Zhong-Wei2,WANG Bao-Lan2 1Department of Neurology,2Department of Rehabilitation,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China |
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| Abstract: | Aim: To investigate a simple method that can establish a reliable rat model of spinal cord injury. Methods: Moderate and severe spinal cord injury models were established by using a modified weight drop device. The pathological and functional changes of the rats in two groups were observed by means of BBB scoring and staining with hematoxylin-eosin(HE) so as to estimate the method was reliable. Results: The BBB scoring showed that the moter function of the rats in two groups recovered gradually over time af... |
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| Keywords: | rat spinal cord injury model animal |
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