靶向EGFR基因的shRNA对胰腺癌细胞放疗敏感性的增强作用

目的 探讨表皮生长因子受体(EGFR)基因对胰腺癌细胞放疗敏感性的增强作用.方法 构建针对EGFR基因序列特异性shRNA的表达载体,用脂质体转染胰腺癌PANC-1细胞,G418筛选建立稳定克隆,采用RT-PCR、Western印迹检测EGFR基因的抑制情况.采用不同剂量X线照射瘤细胞后,用MTT法检测细胞凋亡率,集落形成实验检测细胞集落形成能力,观察胰腺癌细胞对放疗的敏感性.结果 靶向EGFR的序列特异性shRNA可明显抑制EGFR基因的表达,EGFR mRNA和蛋白的抑制率分别为76.3%和71.4%;细胞凋亡增加(P<0.01),克隆形成率减少(P<0.01).结论 体外实验证实靶向EGFR的序列特异性shRNA可明显提高胰腺癌的放疗敏感性.

Enhancement radiosensitivity of pancreatic cancer celIs by shRNA targeting on EGFR

Objective To investigate the enhancement effects of epidermal growth factor receptor(EGFR) gene on the radiosensitivity of pancreatic cancer cells.Methods EGFR gene sequence-specific shRNA expression vector was constructed by lipofectamine transferring pancreatic cancer PANC-1 cells and stable clones was selected by G418,the expression of EGFR gene was detected by RT-PCR and Western blot.The pancreatic cancer cells was irradiated by different doses of X-ray,the radiosensitivity of pancreatic cancer cells wa...