一种改良的胚胎大鼠颞叶海马区神经元的培养方法

目的:探索胚胎大鼠颞叶海马区神经元的改良培养方法。方法:在无血清培养基础上,采用改良的分离纯化法获取单细胞悬液,接种后在不同阶段通过加入不同配方的培养基进行培养,并进行免疫组化鉴定。结果:用该方法培养的颞叶海马区神经元细胞存活率高,生长状态良好,且βⅢ-tublin免疫染色为阳性,神经元细胞纯度可达90%以上。结论:改良分离、结合分阶段的不同培养条件是一种简单、高效的颞叶海马区神经元的纯化培养方法。

A Modified Method for Culture of Neurons from Temporal Lobe and Hippocampal Regions in Embryo Rats

Aim： To explore the modified method for culture of temporal lobe and hippocampal neurons derived from embryo rats. Methods： The single-cell suspension was acquired by modified separated and purified method. The cells were then plated and cultured with different media during different time and identified by immunochemical staining. Results： The neurons from temporal lobe and hippocampal regions exhibited positive staining of bⅢ-tublin, which had super survival rate and good conditions during growth. The purification of isolated neurons can reach over 90%. Conclusion： High purification of neurons can be acquired by modified separation combined with different cultural media during the different phases, so it is a simple and high effective method for the culture of temporal lobe and hippocampal neurons.