| 人卵颗粒细胞凋亡对卵母细胞发育潜能的影响 |
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| 引用本文： | 曹义娟. 人卵颗粒细胞凋亡对卵母细胞发育潜能的影响[J]. 中国妇幼保健, 2012, 27(30): 4780-4782 |
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| 作者姓名： | 曹义娟 |
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| 作者单位： | 东南大学医学院附属 徐州医院江苏省徐州市中心医院妇产科 221009 |
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| 摘 要： | 目的:研究人卵母细胞发育潜能与颗粒细胞凋亡之间的关系。方法:将卵子在体外经过28～30 h培养后,取其颗粒细胞,用TUNEL法进行凋亡率的分析,并与卵子的成熟情况和卵子的评分及形成胚胎结果进行对照。结果:培养后,随着卵母细胞成熟度的降低,凋亡率升高;而且随着卵母细胞评分和发育潜能的降低,颗粒细胞凋亡率逐渐升高。结论:卵子发育潜能与颗粒细胞凋亡之间关系密切,通过测定颗粒细胞的凋亡率可预测未成熟卵母细胞的发育潜能。
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| 关 键 词： | 颗粒细胞 细胞凋亡 卵母细胞 体外成熟 |
Effect of apoptosis of granular cells on developmental potential of human oocytes |
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| CAO Yi-Juan. Effect of apoptosis of granular cells on developmental potential of human oocytes[J]. Maternal and Child Health Care of China, 2012, 27(30): 4780-4782 |
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| Authors: | CAO Yi-Juan |
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| Affiliation: | CAO Yi-Juan.Department of Gynecology and Obstetrics,Xuzhou Hospital Affiliated to Medical College,Southeast University,Central Hospital of Xuzhou City,Xuzhou 221009,Jiangsu,China |
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| Abstract: | Objective:To explore the relationship between developmental potential of human oocytes and apoptosis of granular cells. Methods:After culturing oocytes in vitro for 28-30 hours,the granular cells were obtained,TUNEL method was used to analyze the apoptotic rate,then the results were compared with the maturation situation of oocytes,the score of oocytes,and the embryonic outcome. Results:After culture,the apoptotic rate of granular cells increased with the decrease of maturation degree of oocytes,the decrease of oocytes score,and the decrease of developmental potential of oocytes. Conclusion:The developmental potential of oocytes is closely related to apoptosis of granular cells,the developmental potential of immature oocytes can be predicted by measuring the apoptotic rate of granular cells. |
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| Keywords: | Granular cell Apoptosis Oocyte In vitro maturation |
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